Recombinant human PHKG1 protein (ab101715)
Key features and details
- Expression system: Baculovirus
- Purity: > 75% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human PHKG1 protein -
生理活性
The specific activity of PHKG1 was determined to be 29 nmol/min/mg.
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精製度
> 75 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MTRDEALPDSHSAQDFYENYEPKEILGRGVSSVVRRCIHKPTSQEYAVKV IDVTGGGSFSPEEVRELREATLKEVDILRKVSGHPNIIQLKDTYETNTFF FLVFDLMKRGELFDYLTEKVTLSEKETRKIMRALLEVICTLHKLNIVHRD LKPENILLDDNMNIKLTDFGFSCQLEPGERLREVCGTPSYLAPEIIECSM NEDHPGYGKEVDMWSTGVIMYTLLAGSPPFWHRKQMLMLRMIMSGNYQFG SPEWDDYSDTVKDLVSRFLVVQPQNRYTAEEALAHPFFQQYLVEEVRHFS PRGKFKVIALTVLASVRIYYQYRRVKPVTREIVIRDPYALRPLRRLIDAY AFRIYGHWVKKGQQQNRAALFENTPKAVLLSLAEEDY -
予測される分子量
70 kDa including tags -
領域
1 to 387
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関連製品
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab101715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
ab204885 (ZIP Kinase peptide substrate) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- PHK gamma M
- PHK-gamma-M
- Phkg
see all -
機能
Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
ドメイン
The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains. - Information by UniProt
画像
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Functional Studies - Recombinant human PHKG1 protein (ab101715)The specific activity of PHKG1 (ab101715) was determined to be 26 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human PHKG1 protein (ab101715)SDS PAGE analysis of ab101715 -
SDS-PAGE - Recombinant human PHKG1 protein (ab101715)The putity of ab101715 was determined to be 75% by densitometry.
Approximate MWt: 70kDa -
Functional Studies - Recombinant human PHKG1 protein (ab101715)The specific activity of ab101715 was determined to be 29 nmol/min/mg.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab101715 は論文での使用が確認できていません。
