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  1. Link

    recombinant-human-pdgf-b-protein-ab9706.pdf

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Cardiovascular Blood Serum Proteins
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Recombinant human PDGF B protein (ab9706)

  • Datasheet
Submit a review Q&A (7)References (1)

Product price, shipping and contact information

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出荷および受注について

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Achieve higher consistency and quality standards with a premium grade bioactive protein

Product image
Recombinant human PDGF B protein (Active) (ab259425)
  • High batch-to-batch consistency
  • Optimal bioactivity
  • Guaranteed identical to human native proteins
  • >95% purity
  • Ultra-low endotoxin levels: <0.005 Eu/µg
  • Carrier and tag free

製品の詳細

  • 製品名

    Recombinant human PDGF B protein
    PDGF B タンパク質・ペプチド 製品一覧
  • 精製度

    > 95 % SDS-PAGE.
    Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • 発現系

    Escherichia coli
  • アクセッション番号

    P01127
  • タンパク質長

    Full length protein
  • Animal free

    No
  • 由来

    Recombinant
    • 生物種

      Human
    • 配列

      SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
    • 予測される分子量

      16 kDa
    • 配列の追加情報

      This is the full length mature form of PDGF B

関連製品

  • Related Products

    • Anti-PDGF BB antibody (ab9704)

特性

Our Abpromise guarantee covers the use of ab9706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態

    Lyophilized
  • 備考

    Biological Activity is determined by the dose-dependent stimulation of the proliferation of BALB/c 3T3 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Reconstitute with water to a concentration of 0.1 – 1 mg/ml. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. If reconstituting to a concentration less than 0.1 mg/ml add a carrier protein such as 0.1% BSA.

関連情報

  • 別名

    • Becaplermin
    • c sis
    • FLJ12858
    • Oncogene SIS
    • PDGF 2
    • PDGF B chain
    • PDGF Beta
    • PDGF subunit B
    • PDGF-2
    • PDGF2
    • Pdgfb
    • PDGFB_HUMAN
    • Platelet derived growth factor 2
    • Platelet derived growth factor B chain
    • Platelet derived growth factor beta
    • Platelet derived growth factor beta polypeptide
    • Platelet derived growth factor beta polypeptide (oncogene SIS)
    • Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog)
    • Platelet-derived growth factor B chain
    • Platelet-derived growth factor beta polypeptide
    • Platelet-derived growth factor subunit B
    • Proto-oncogene c-Sis
    • Simian sarcoma viral (v sis) oncogene homolog
    • SIS
    • SSV
    • v sis platelet derived growth factor beta polypeptide
    see all
  • Target information above from: UniProt accession P01127 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (1)

ab9706 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9706 は 1 報の論文で使用されています。

  • Riessland M  et al. Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons. Cell Stem Cell 25:514-530.e8 (2019). PubMed: 31543366

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-7 of 7 Abreviews or Q&A

Question

ab84265 is detection Ab
which capture Ab was used?

Read More

Abcam community

Verified customer

Asked on Jan 23 2013

Answer


The lab let me know that this antibody (ab84265, detection antibody) can be used with ab9704 as capture antibody and ab9706 as protein standard.

Read More

Abcam Scientific Support

Answered on Jan 23 2013

Question

more details on functional studies donewith ab73229 and ab9706?

Read More

Abcam community

Verified customer

Asked on Aug 16 2012

Answer

Thank you for contacting us.

For ab73229, I was not able to receive any additional information for using the protein infunctional studies. As for ab9706, the procedure is as follows:

Procedure: (Previously reconstituted in water and stored at -20oC in 1XPBS+0.1%BSA)

1) Resuspend 3T3 cells in regular culture media with 10% calf serum. Transfer 100ml culture/well to assay plate (5,000 cells/well; passage # 7). Incubate overnight.

2) Complete media change with 100ml assay media containing 0.5% calf serum. Incubate overnight.

3) Complete media change with assay media.

4) Serial dilute h-PDGF-BB in assay media.

5) Transfer 100ml diluted cytokine to cells in assay plate. Volume of each well is 200ml, containing 0.5% calf serum and h-PDGF-BB as indicated below.

6) Incubate 42 hours.

7) Add 20ml of "other company" Substrate Cell Titer 96 Aqueous One Solution Reagent to each well.

8) Incubate 37oC and read at OD 490nm.


In direct comparison, ab9706 is a highly pure (>98%) recombinant human PDGF-BB (Platelet-Derived Growth Factor-BB), Expression host: E. coli, while ab73229 is arecombinant Full Length human PDGF BB is a homodimeric, glycosylated, polypeptide chain containing 109 (X 2) amino acids and having a molecular mass of 32 kDa, purified from Saccharomyces cerevisiae.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Aug 16 2012

Question

Hi, Thanks for that, I just have one remaining question about its reconstitution, by carrier protein do you mean Human or bovine serum albumin as stated for the reconstition of ab51925? Help on this would be most useful

Read More

Abcam community

Verified customer

Asked on Oct 17 2011

Answer

Yes, either HSA or BSA will be fine to use in the reconstitution of ab9706. We would also recommend a quick spin in a microcentrifuge prior to opening the vial to make sure the protein gets down to the bottom of the vial, then using sterile H2O and adding a carrier protein as mentioned if diluting below 0.1mg/ml (for example 0.1% BSA). If you have any further questions please do not hesitate to ask.

Read More

Abcam Scientific Support

Answered on Oct 17 2011

Question

Hi, I am a researcher at Edinburgh University and I have just ordered and received PDGF BB protein (ab9706) for use in growth medium Purchase Order Number 4506313210/50F. Upon looking for its reconstitution I have discovered another PDGF BB protein (ab51925) with the abbreviation (Active). Can PDGF BB protein ab9706 be used for my purpose or is it inactive? If so I can return the Inactive form and have it replaced with the active form as the inactive form is of no use for these purposes. I think that if this is inactive, it should have been made clear on the website otherwise I would clearly not have purchased this. I wish to commence a series of very expensive experiments which will require continued use of this growth factor, could you please clarify this as soon as possible,

Read More

Abcam community

Verified customer

Asked on Oct 14 2011

Answer

Thank you for contacting us. Both PDGF BB protein ab9706 (human) and ab51925 (mouse) have been assessed for activity by dose-dependent stimulation of the proliferation of Balb/c 3T3 cells with ED50 of 1.0-3.0 ng/ml and 2 ng/ml resepectively. They are both active. I am sorry that the information provided on the website has been confusing and thank you for bringing this to our attention. I will be amending this for future customers. ab9706 can be reconstitution with water, however a carrier protein is required if aliquotting under 0.1mg/ml. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Oct 14 2011

Question

How should this product be reconstituted?

Read More

Abcam community

Verified customer

Asked on May 17 2007

Answer

Thank you for your enquiry. Human PDGF-BB, ab9706, is reconstituted in water: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. Please do not hesitate to contact me should you have any additional questions.

Read More

Abcam Scientific Support

Answered on May 17 2007

Question

What are the reconstitution instructions for this protein? I was also wondering if I need to centrifuge it before opening it. I apperciate your help in advance. Thanks,

Read More

Abcam community

Verified customer

Asked on Apr 27 2007

Answer

Thank you for your enquiry. Yes, we would highly recommend centrifuging the vial before opening, as some of the contents may have attached to the cap region during transport. To reconstitute this product, you can dilute in water. The volume of water depends on the concentration of the product itself, which is 10ug. Then, you can dilute the product in any other buffer in order to place it in working aliquots. I hope this information will be useful. Should you require any further assistance, please do not hesitate to contact me.

Read More

Abcam Scientific Support

Answered on Apr 27 2007

Question

Can PDGF-BB be diluted in water? If not, what can I dilute it in?

Read More

Abcam community

Verified customer

Asked on Apr 13 2005

Answer

Thank you for your enquiry. Yes, this product can be diluted water. The volume of water depends on the concentration of the product itself. Then, you can dilute the product in any other buffer in order to place it in working aliquots. Have you purchased this product? If so, could you please reply with the lot number which is on the vial label?

Read More

Abcam Scientific Support

Answered on Apr 13 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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