Recombinant human PDGF AA protein (Animal Free) (ab179505)
Key features and details
- Expression system: Escherichia coli
- Purity: > 97% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
-
製品名
Recombinant human PDGF AA protein (Animal Free)
PDGF AA タンパク質・ペプチド 製品一覧 -
生理活性
The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically 3-5 ng/mL.
-
精製度
> 97 % SDS-PAGE. -
エンドトキシン・レベル
<=1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
Yes -
由来
Recombinant -
-
生物種
Human -
配列
SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT -
予測される分子量
29 kDa -
領域
87 to 211 -
配列の追加情報
ab179505 is a non-glycosylated disulfide-linked homodimer, containing two 125 amino acid chains.
-
特性
Our Abpromise guarantee covers the use of ab179505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
-
製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
関連情報
-
別名
- PDGF A
- PDGF A chain
- PDGF subunit A
see all -
機能
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. -
配列類似性
Belongs to the PDGF/VEGF growth factor family. -
ドメイン
The long form contains a basic insert which acts as a cell retention signal. -
細胞内局在
Secreted. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab179505 は論文での使用が確認できていません。