Recombinant human PDE6C protein (Active) (ab271682)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 5% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human PDE6C protein (Active)
PDE6C タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: ≥ 3,000 pmol/min/µg.Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cGMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE6C at 37°C for 60 min. Quantified by 5'- nucleotidase cleaving the 5' -GMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
精製度
>= 5 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
125 kDa including tags -
領域
1 to 855 -
タグ
GST tag N-Terminus -
配列の追加情報
Mature chain.
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特性
Our Abpromise guarantee covers the use of ab271682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine), 0.06% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- 5''-cyclic phosphodiesterase subunit alpha''
- cGMP phosphodiesterase 6C
- COD4
see all -
関連疾患
Defects in PDE6C are the cause of cone dystrophy type 4 (COD4) [MIM:613093]. An early-onset cone dystrophy. Cone dystrophies are retinal dystrophies characterized by progressive degeneration of the cone photoreceptors with preservation of rod function, as indicated by electroretinogram. However, some rod involvement may be present in some cone dystrophies, particularly at late stage. Affected individuals suffer from photophobia, loss of visual acuity, color vision and central visual field. Another sign is the absence of macular lesions for many years. Cone dystrophies are distinguished from the cone-rod dystrophies in which some loss of peripheral vision also occurs. -
配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
細胞内局在
Cell membrane. - Information by UniProt
画像
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Functional Studies - Recombinant human PDE6C protein (Active) (ab271682)
Specific activity of ab271682 was ≥ 3,000 pmol/min/µg.
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SDS-PAGE - Recombinant human PDE6C protein (Active) (ab271682)SDS-PAGE analysis of 2 µg ab271682.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271682 は論文での使用が確認できていません。
