Recombinant human PD1 protein (Tagged) (Biotin) (ab271670)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, DDDDK tag C-Terminus, Avi tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human PD1 protein (Tagged) (Biotin)
PD1 タンパク質・ペプチド 製品一覧 -
生理活性
Assay Conditions: Briefly, 50 µl of 2 µg/ml PD-L1 was coated onto plates. 20 µl of 0.5 µg/ml PD-1, FLAG-His, Biotin-labelled was allowed to bind at room temperature for 2 hours. Strep-HRP was added and allowed to incubate at room temp for 1 hr, followed by HPR chemiluminescent substrate addition and plate reading.
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精製度
>= 80 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
LDSPDRPWNP PTFSPALLVV TEGDNATFTC SFSNTSESFV LNWYRMSPSN QTDKLAAFPE DRSQPGQDCR FRVTQLPNGR DFHMSVVRAR RNDSGTYLCG AISLAPKAQI KESLRAELRV TERRAEVPTA HPSPSPRPAG QFQ -
予測される分子量
20 kDa including tags -
分子量情報
This protein runs at a higher molecular weight by SDS-PAGE due to glycosylation. -
領域
25 to 167 -
修飾
biotinylated -
タグ
His tag C-Terminus , DDDDK tag C-Terminus , Avi tag C-Terminus
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標識
Biotin
関連製品
特性
Our Abpromise guarantee covers the use of ab271670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
Enzymatically biotin-labeled using Avi-tag™ technology
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- CD279
- CD279 antigen
- hPD 1
see all -
機能
Possible cell death inducer, in association with other factors. -
関連疾患
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
発生段階
Induced at programmed cell death. -
細胞内局在
Membrane. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human PD1 protein (Tagged) (Biotin) (ab271670)SDS-PAGE analysis of ab271670.
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Functional Studies - Recombinant human PD1 protein (Tagged) (Biotin) (ab271670)Binding activity of ab271670.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271670 は論文での使用が確認できていません。