Recombinant human PCSK9 protein (Active) (Biotin) (ab271653)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, Avi tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human PCSK9 protein (Active) (Biotin)
PCSK9 タンパク質・ペプチド 製品一覧 -
生理活性
50 µL reaction mix (50 mM HEPES pH 7.4, 50 mM NaCl, 200 µM CaCl2, 0.05% Tween-20, 0.1% BSA, and 0–1500 ng biotin-labeled PCSK9) is added to wells precoated with LDLR Ectodomain. Incubate for 2 hr. Add streptavidin-labeled HRP and incubate 1 hour. Finally, add HRP chemiluminescent substrates and read luminescence.
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精製度
>= 80 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
QEDEDGDYEELVLALRSEEDGLAEAPEHGTTATFHRCAKDPWRLPGTYVV VLKEETHLSQSERTARRLQAQAARRGYLTKILHVFHGLLPGFLVKMSGDL LELALKLPHVDYIEEDSSVFAQSIPWNLERITPPRYRADEYQPPDGGSLV EVYLLDTSIQSDHREIEGRVMVTDFENVPEEDGTRFHRQASKCDSHGTHL AGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPV GPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFRDDACLYSPASA PEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFV SQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFP EDQRVLTPNLVAALPPSTHGAGWQLFCRTVWSAHSGPTRMATAVARCAPD EELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEGVYAIARCCLLP QANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLR PRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGW TLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEGAVTAVAICCR SRHLAQASQELQGGGLNDIFEAQKIEWHEHHHHHH -
予測される分子量
74 kDa -
領域
31 to 692 -
タグ
His tag C-Terminus , Avi tag C-Terminus -
配列の追加情報
Signal peptide removed.
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標識
Biotin
関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab271653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
Enzymatically biotin-labeled using Avi-tag™ technology
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 8.00
Constituents: 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.48% TrisThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Convertase subtilisin/kexin type 9 preproprotein
- FH3
- HCHOLA3
see all -
機能
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. -
組織特異性
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. -
関連疾患
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant. -
配列類似性
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain. -
翻訳後修飾
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271653 は論文での使用が確認できていません。