Recombinant human PARP7 protein (Tagged) (ab271651)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 33% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human PARP7 protein (Tagged) -
生理活性
Specific Activity: >40 pmol/min/μg
Assay Conditions: Assay was done according to PARP7 assay kit protocol. First, histone proteins are coated on a 96-well plate. Next, the biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
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精製度
>= 33 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
KRRPLFRSCF ILLPYLQTLG GVPTQAPPPL EATSSSQIIC PDGVTSANFY PETWVYMHPS QDFIQVPVSA EDKSYRIIYN LFHKTVPEFK YRILQILRVQ NQFLWEKYKR KKEYMNRKMF GRDRIINERH LFHGTSQDVV DGICKHNFDP RVCGKHATMF GQGSYFAKKA SYSHNFSKKS SKGVHFMFLA KVLTGRYTMG SHGMRRPPPV NPGSVTSDLY DSCVDNFFEP QIFVIFNDDQ SYPYFVIQYE EVSNTVSI -
予測される分子量
31 kDa including tags -
領域
400 to 657 -
タグ
DDDDK tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab271651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
100 µg/mL DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- ADP-ribosyltransferase diphtheria toxin-like 14
- ARTD14
- AW558171
see all -
機能
Poly [ADP-ribose] polymerase using NAD(+) as a substrate to transfer ADP-ribose onto glutamic acid residues of a protein acceptor; repeated rounds of ADP-ribosylation leads to the formation of poly(ADPribose) chains on the protein, thereby altering the function of the target protein. May play a role in the adaptative response to chemical exposure (TCDD) and thereby mediates certain effects of the chemicals. -
配列類似性
Contains 1 C3H1-type zinc finger.
Contains 1 PARP catalytic domain.
Contains 1 WWE domain. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human PARP7 protein (Tagged) (ab271651)
SDS-PAGE analysis of ab271651.
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Functional Studies - Recombinant Human PARP7 protein (Tagged) (ab271651)Specific activity of ab271651 was >40 pmol/min/µg.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271651 は論文での使用が確認できていません。
