Recombinant Human Papillomavirus 16 (E7) protein (His tag) (ab237790)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
製品の詳細
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製品名
Recombinant Human Papillomavirus 16 (E7) protein (His tag) -
精製度
> 90 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human papillomavirus -
配列
MHGDTPTLHEYMLDLQPETTDLYCYEQLNDSSEEEDEIDGPAGQAEPDRA HYNIVTFCCKCDSTLRLCVQSTHVDIRTLEDLLMGTLGIVCPICSQKP -
予測される分子量
15 kDa including tags -
領域
1 to 98 -
タグ
His tag N-Terminus
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製品の詳細
Recombinant Human papillomavirus Human Papillomavirus 16 (E7) protein (His tag)
関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab237790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Mass Spectrometry
SDS-PAGE
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質量分析
LC-MS/MS -
製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.20
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
関連情報
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別名
- E7
- HPV16 E7
- Protein E7
- Transforming protein
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関連性
E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription -
細胞内局在
Nuclear
画像
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab237790.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab237790 could indicate that this peptide derived from E.coli-expressed Human Papillomavirus 16 (E7).
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab237790 could indicate that this peptide derived from E.coli-expressed Human Papillomavirus 16 (E7).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab237790 は論文での使用が確認できていません。