Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品名Recombinant human PAI1 (mutated S338) protein
PAI1 タンパク質・ペプチド 製品一覧
Specific activity: > 95 percent active by uPA titration; 1 mole NBD / mole PAI1. Excitation: 490 nm; Emission profile: 520 nm – 600 nm. Binding of uPA or tPA results in increase of peak at 540 nm.
精製度> 95 % SDS-PAGE.
ab92773 is > 98% pure by SDS-PAGE.
タンパク質長Full length protein
Our Abpromise guarantee covers the use of ab92773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
備考Protect from light.
Solubility: > 2 mg/mL and < 5 mg/mL
ab92773 was created by mutagenesis of the P9 serine residue (Ser338) on the reactive center loop to cysteine. This then provided a free thiol group for incorporation of N,N'-dimethyl-N-(acetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) (NBD), a fluorescent probe highly sensitive to changes in solvation and hydrophobic environment. The fluorescence emission of P9-NBD PAI1 is enhanced 6-7 fold upon insertion of the reactive center loop into beta-sheet A following complex formation with proteinases, formation of the latent species, or cleavage by elastase. The incorporated probe is excited at 480 nm and displays a broad emission spectrum with a peak centered 542 nm with a resultant blue-shift to 520 nm following reactive center loop insertion. The modified PAI1 is nearly as active as wt PAI-1 and is more resistant to the spontaneous latency reaction making this an excellent tool for monitoring reaction rates of PAI1. ab92773 has been utilized in a number of studies to determine the rates of loop insertion and SERPIN reaction mechanisms when reacted with various proteinases, inactivating antibodies and conformational changes imposed by the binding of vitronectin.
Concentration information loading...
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Clade E
- Endothelial plasminogen activator inhibitor
機能This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
組織特異性Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
関連疾患Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
配列類似性Belongs to the serpin family.
翻訳後修飾Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.