Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品名Recombinant human PAI1 (mutated N150H + K154T + Q319L + M354I) protein
PAI1 タンパク質・ペプチド 製品一覧
>95% active by uPA titration. Kinetic Data: Second order rate constants for inhibition of: uPA = 5.1 X 106 M-1s-1 tPA = 7.9 X 105 M-1s-1
精製度> 95 % SDS-PAGE.
> 99% pure by SDS PAGE
タンパク質長Full length protein
Our Abpromise guarantee covers the use of ab92969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
備考Solubility: > 2 mg/mL and < 5 mg/mL Ultraviolet: Absorbance (280nm) = 2.5 epsilon 0.1% = 1.0
ab92969 contains the following four mutations: K154T, Q319L, M354I and N150H. These mutations combine to confer great stability to the otherwise labile molecule essentially locking in into the active conformation. It is an ideal choice for in vivo studies.
Concentration information loading...
Shipped on Dry Ice. Store at -80°C.
Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Clade E
- Endothelial plasminogen activator inhibitor
機能This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
組織特異性Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
関連疾患Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
配列類似性Belongs to the serpin family.
翻訳後修飾Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.