Recombinant human p35 + CDK5 protein (Tagged) (ab271460)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 60% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant human p35 + CDK5 protein (Tagged) -
生理活性
Specific acivity: 180 pmol/min/µg
Assay was performed in Kinase buffer containing 0.2 mM DTT using Histone 1 substrate (0.2 mg/ml) and 20 mM ATP. Reaction was performed at 30°C for 45 min.
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精製度
>= 60 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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アミノ酸配列 1
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生物種
Human -
配列
MQKYEKLEKIGEGTYGTVFKAKNRETHEIVALKRVRLDDDDEGVPSSALR EICLLKELKHKNIVRLHDVLHSDKKLTLVFEFCDQDLKKYFDSCNGDLDP EIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLINRNGELKLADFGLARA FGIPVRCYSAEVVTLWYRPPDVLFGAKLYSTSIDMWSAGCIFAELANAGR PLFPGNDVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKPYPMYPATTSLVN VVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYFSDFCPP -
予測される分子量
108 kDa -
領域
1 to 292 -
タグ
GST tag N-Terminus
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アミノ酸配列 2
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生物種
Human -
配列
MGTVLSLSPSYRKATLFEDGAATVGHYTAVQNSKNAKDKNLKRHSIISVL PWKRIVAVSAKKKNSKKVQPNSSYQNNITHLNNENLKKSLSCANLSTFAQ PPPAQPPAPPASQLSGSQTGGSSSVKKAPHPAVTSAGTPKRVIVQASTSE LLRCLGEFLCRRCYRLKHLSPTDPVLWLRSVDRSLLLQGWQDQGFITPAN VVFLYMLCRDVISSEVGSDHELQAVLLTCLYLSYSYMGNEISYPLKPFLV ESCKEAFWDRCLSVINLMSSKMLQINADPHYFTQVFSDLKNESGQEDKKR LLLGLDR -
領域
1 to 307 -
タグ
GST tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab271460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.49% Glutathione, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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細胞内局在
p35: Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites. CDK5: Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia.
画像
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SDS-PAGE - Recombinant Human p35 + CDK5 protein (Tagged) (ab271460)SDS-PAGE analysis of ab271460.
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Functional Studies - Recombinant Human p35 + CDK5 protein (Tagged) (ab271460)Specific activity of ab271460.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271460 は論文での使用が確認できていません。
