Recombinant Human Nucleolin protein (His tag) (ab241527)
Key features and details
- Expression system: Yeast
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
製品の詳細
-
製品名
Recombinant Human Nucleolin protein (His tag) -
精製度
> 90 % SDS-PAGE. -
発現系
Yeast -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
VKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQK KGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPA KAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDD DSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDD EEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDE DDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGT EPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGY VDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLP YKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQG TEIDGRSISLYYTGEKGQNQDYRGGKNSTWS -
予測される分子量
54 kDa including tags -
領域
2 to 482 -
タグ
His tag N-Terminus -
配列の追加情報
N-terminal 6xHis-tagged
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab241527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Mass Spectrometry
SDS-PAGE
-
質量分析
LC-MS/MS -
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.20
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
関連情報
-
別名
- C23
- FLJ45706
- MS1116
see all -
機能
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
配列類似性
Contains 4 RRM (RNA recognition motif) domains. -
翻訳後修飾
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
画像
-
ab241527 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
-
Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of ab241527 could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) Nucleolin.
-
Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of ab241527 could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) Nucleolin.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab241527 は 1 報の論文で使用されています。
- Mulvaney KM et al. Molecular basis for substrate recruitment to the PRMT5 methylosome. Mol Cell 81:3481-3495.e7 (2021). PubMed: 34358446