Recombinant human NGF protein (Active) (ab9796)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human NGF protein (Active)
NGF タンパク質・ペプチド 製品一覧 -
生理活性
Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.
The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
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精製度
>= 98 % SDS-PAGE.
>=98% HPLC analyses. Sterile filtered. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA -
予測される分子量
27 kDa -
領域
122 to 241 -
配列の追加情報
Full length mature protein, without the signal peptide or the propeptide. The functional form is a non-covalently linked homodimer 27 kDa.
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特性
Our Abpromise guarantee covers the use of ab9796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Please reconstitute this product to 0.1-1 mg/ml in water. Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA) for long term storage.
関連情報
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別名
- Beta nerve growth factor
- Beta NGF
- Beta-nerve growth factor
see all -
機能
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). -
関連疾患
Neuropathy, hereditary sensory and autonomic, 5 -
配列類似性
Belongs to the NGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
画像
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Western blot - Recombinant human NGF protein (ab9796)All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/1000 dilution
Lane 1 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 2 : PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab9796 は論文での使用が確認できていません。
