Bioactive grade

Recombinant human NGF protein (Active) (ab9796)

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製品の詳細

  • 製品名

    Recombinant human NGF protein (Active)
    NGF タンパク質・ペプチド 製品一覧

  • 生理活性

    Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.

    The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

  • 精製度

    >= 98 % SDS-PAGE.
    >=98% HPLC analyses. Sterile filtered.

  • エンドトキシン・レベル

    < 1.000 Eu/µg

  • 発現系

    Escherichia coli

  • アクセッション番号

    P01138

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRASSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA

    • 予測される分子量

      27 kDa

    • 領域

      122 to 241

    • 配列の追加情報

      Full length mature protein, without the signal peptide or the propeptide. The functional form is a non-covalently linked homodimer 27 kDa.

関連製品

    特性

    Our Abpromise guarantee covers the use of ab9796 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      HPLC

      Functional Studies

      SDS-PAGE

    • 製品の状態

      Lyophilized
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      n/a

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • 再構成
      Please reconstitute this product to 0.1-1 mg/ml in water. Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA) for long term storage.

    関連情報

    • 別名

      • Beta nerve growth factor
      • Beta NGF
      • Beta-nerve growth factor
      • Beta-NGF
      • HSAN5
      • MGC161426
      • MGC161428
      • Nerve growth factor
      • Nerve growth factor (beta polypeptide)
      • Nerve growth factor beta
      • Nerve growth factor beta polypeptide
      • Nerve growth factor beta subunit
      • NGF
      • NGF_HUMAN
      • NGFB
      • NID67
      see all

    • 機能

      Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).

    • 関連疾患

      Neuropathy, hereditary sensory and autonomic, 5

    • 配列類似性

      Belongs to the NGF-beta family.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    画像

    • Western blot - Recombinant human NGF protein (ab9796)
      Western blot - Recombinant human NGF protein (ab9796)
      All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/1000 dilution

      Lane 1 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 2 : PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 140 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab9796 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9796 は論文での使用が確認できていません。

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Question

    Is the weight (20 ug) determined from the monomer or dimer form of the protein?

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    Answer

    Ab9796 is a noncovalently disulfide-linked homodimer, of two 13.5kDa polypeptide monomers (238 total amino acid residues). The three disulfide bonds are required for biological activity.

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    Question

    I would like to purchase 6 vials of this protein. What is the lead time? and if I wanted to purchase a certain amount every week on a standing order, is this possible? I plan to use 20 ug per animal per delivery (6 times over 2 weeks) and I would like to guarantee delivery.

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    Answer

    As you have probably seen from the online datasheet, ab9796 -- NGF beta (human) recombinant protein -- is not currently in stock. The lead time for this item would be approx 7 working days. If you were to order 6 units on a single purchase order I could offer you a 10% discount on the regular price of USD 293 for 20µg. Unfortunately we do not have the facilities to deal with a weekly standing order. I would recommend that you order sufficient quantities of this product for your research and if you require more than 6 units for your whole study, I can investigate the possibility of a larger bulk discount.

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