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Recombinant human Neurotrophin 3 protein (ab9792)

Submit a review Q&A (1)References (1)
Western blot - Recombinant human Neurotrophin 3 protein (ab9792)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 98% SDS-PAGE
    • Active: Yes
    • Suitable for: Functional Studies, SDS-PAGE, WB

    製品の詳細

    • 製品名

      Recombinant human Neurotrophin 3 protein
      Neurotrophin 3 タンパク質・ペプチド 製品一覧

    • 生理活性

      Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.

    • 精製度

      > 98 % SDS-PAGE.
      Sterile filtered Greater than 98% pure by HPLC analyses. Endotoxin level is less than 0.1 g/ml (1EU/g).

    • 発現系

      Escherichia coli

    • アクセッション番号

      P20783

    • タンパク質長

      Protein fragment

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT

      • 領域

        139 to 257

    特性

    Our Abpromise guarantee covers the use of ab9792 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Functional Studies

      SDS-PAGE

      Western blot

    • 製品の状態

      Lyophilized
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • 再構成
      For lot specific reconstitution information please contact our Scientific Support Team.

    関連情報

    • 別名

      • HDNF
      • MGC129711
      • Nerve growth factor 2
      • Neurotrophic factor
      • Neurotrophin 3 precursor
      • Neurotrophin-3
      • Neurotrophin3
      • NGF 2
      • NGF-2
      • NGF2
      • NT 3
      • NT-3
      • NT3
      • NTF 3
      • NTF3
      • NTF3_HUMAN
      see all

    • 機能

      Seems to promote the survival of visceral and proprioceptive sensory neurons.

    • 組織特異性

      Brain and peripheral tissues.

    • 配列類似性

      Belongs to the NGF-beta family.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    画像

    • Western blot - Recombinant human Neurotrophin 3 protein (ab9792)
      Western blot - Recombinant human Neurotrophin 3 protein (ab9792)
      Anti-Neurotrophin 3 antibody (ab65804) at 1 µg/ml + Recombinant human Neurotrophin 3 protein (ab9792) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 30 seconds

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab9792 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9792 は 1 報の論文で使用されています。

    • Mantuano E  et al. The Unfolded Protein Response Is a Major Mechanism by Which LRP1 Regulates Schwann Cell Survival after Injury. J Neurosci 31:13376-85 (2011). PubMed: 21940431

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    This neural growth factor is lyophilized. It is correct that it has to be resuspended in MQ water?



    Sincerely,

    Read More
    Answer

    Thank you for contacting us.


    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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