Recombinant human NEK11 protein (ab125544)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human NEK11 protein -
生理活性
The specific activity of ab125544 was determined to be 25 nmol/min/mg as per activity assay protocol.
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精製度
> 90 % SDS-PAGE.
Assessed by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
110 kDa including tags -
領域
1 to 645
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関連製品
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab125544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.004% EGTA, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- 4932416N14Rik
- FLJ23495
- NEK11
see all -
機能
Possible role in S-phase checkpoint mechanism. -
組織特異性
Poorly expressed in cerebellum, trachea, lung, appendix, and uterus. -
配列類似性
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
発生段階
Predominantly expressed at S/G2/M phase. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Nucleus. Nucleus > nucleolus. Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab125544 は論文での使用が確認できていません。