Recombinant human MAP3K1 protein (ab104027)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human MAP3K1 protein -
生理活性
The Specific activity of ab104027 was determined to be 820 nmol/min/mg.
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精製度
> 75 % SDS-PAGE.
Purity was determined to be >75% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
155 kDa including tags -
領域
664 to 1512
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関連製品
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab104027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
ab126919 (Human MEK1 full length protein), ab126906 (Human ERK1 full length protein) and ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as MEKK1
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.01% Triton-X-100, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- M3K1_HUMAN
- MAP3K1
- MAPK/ERK kinase kinase 1
see all -
機能
Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 RING-type zinc finger.
Contains 1 SWIM-type zinc finger. -
翻訳後修飾
Autophosphorylated. - Information by UniProt
画像
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The specific activity of MAP3K1 (ab104027) was determined to be 8.5 nmol/min/mg as per activity assay protocol and was equivalent to 820 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab104027
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SDS PAGE analysis of ab104027
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Kinase Assay demonstrating specific activity of ab104027.
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SDS-PAGE showing ab104027 at approximately 155kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab104027 は論文での使用が確認できていません。