Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB
製品名Recombinant Human Lipoamide Dehydrogenase protein
Specific activity is > 200 units/mg, one unit will reduce 1.0 umole of DL-lipoamide to DL-dihydrolipoamide per minute at pH 6.5 at 25C.
精製度> 95 % SDS-PAGE.
ab93679 is purified using conventional chromatography techniques.
タンパク質長Full length protein
Our Abpromise guarantee covers the use of ab93679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Dehydrogenase complex, E3 component
- Dihydrolipoamide dehydrogenase
機能Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
関連疾患Note=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
配列類似性Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.