Recombinant human KMT1E / SETDB1 protein (Active) (ab271738)

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Functional Studies - Recombinant human KMT1E / SETDB1 protein (Active) (ab271738)
  • SDS-PAGE - Recombinant human KMT1E / SETDB1 protein (Active) (ab271738)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: >= 48% SDS-PAGE
  • Active: Yes
  • Tags: His tag N-Terminus, GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

製品の詳細

  • 製品名

    Recombinant human KMT1E / SETDB1 protein (Active)
    KMT1E / SETDB1 タンパク質・ペプチド 製品一覧

  • 生理活性

    Assay was performed using a SETDB1 Chemiluminescent Assay Kit.

  • 精製度

    >= 48 % SDS-PAGE.

  • 発現系

    Baculovirus infected Sf9 cells

  • アクセッション番号

    Q15047

  • タンパク質長

    Protein fragment

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      ALSENVSGGKPGINQTYRSPLGSTASAPAPSALPAPPAPPVFHGMLERAP AEPSYRAPMEKLFYLPHVCSYTCLSRVRPMRNEQYRGKNPLLVPLLYDFR RMTARRRVNRKMGFHVIYKTPCGLCLRTMQEIERYLFETGCDFLFLEMFC LDPYVLVDRKFQPYKPFYYILDITYGKEDVPLSCVNEIDTTPPPQVAYSK ERIPGKGVFINTGPEFLVGCDCKDGCRDKSKCACHQLTIQATACTPGGQI NPNSGYQYKRLEECLPTGVYECNKRCKCDPNMCTNRLVQHGLQVRLQLFK TQNKGWGIRCLDDIAKGSFVCIYAGKILTDDFADKEGLEMGDEYFANLDH IESVENFKEGYESDAPCSSDSSGVDLKDQEDGNSGTEDPEESNDDSSDDN FCKDEDFSTSSVWRSYATRRQTRGQKENGLSETTSKDSHPPDLGPPHIPV PPSIPVGGCNPPSSEETPKNKVASWLSCNSVSEGGFADSDSHSSFKTNEG GEGRAGGSRMEAEKASTSGLGIKDEGDIKQAKKEDTDDRNKMSVVTESSR NYGYNPSPVKPEGLRRPPSKTSMHQSRRLMASAQSNPDDVLTLSSSTESE GESGTSRKPTAGQTSATAVDSDDIQTISSGSEGDDFEDKKNMTGPMKRQV AVKSTRGFALKSTHGIAIKSTNMASVDKGESAPVRKNTRQFYDGEESCYI IDAKLEGNLGRYLNHSCSPNLFVQNVFVDTHDLRFPWVAFFASKIRAGTE LTWDYNYEVGSVEGKELLCCCGAIECRGRLL

    • 予測される分子量

      114 kDa including tags

    • 領域

      510 to 1290

    • タグ

      His tag N-Terminus , GST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab271738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態

    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.12% Glutathione

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名

    • AU022152
    • EC 2.1.1.43
    • ERG-associated protein with SET domain
    • ESET
    • H3 K9 HMTase 4
    • H3-K9-HMTase 4
    • H3-K9-HMTase4
    • Histone H3 K9 methyltransferase 4
    • Histone H3-K9 methyltransferase 4
    • Histone-lysine N-methyltransferase SETDB1
    • KG1T
    • KIAA0067
    • KMT1E
    • Lysine N-methyltransferase 1E
    • MGC90670
    • mKIAA0067
    • SET domain bifurcated 1
    • Set domain protein, bifurcated, 1
    • SETB1_HUMAN
    • Setdb1
    see all

  • 機能

    Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.

  • 組織特異性

    Widely expressed. High expression in testis.

  • 配列類似性

    Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 MBD (methyl-CpG-binding) domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
    Contains 2 Tudor domains.

  • ドメイン

    The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
    Isoform 2 lacks all domains required for histone methyltransferase activity.

  • 細胞内局在

    Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
  • Information by UniProt

画像

  • Functional Studies - Recombinant human KMT1E / SETDB1 protein (Active) (ab271738)
    Functional Studies - Recombinant human KMT1E / SETDB1 protein (Active) (ab271738)
    Specific activity of ab271738.
  • SDS-PAGE - Recombinant human KMT1E / SETDB1 protein (Active) (ab271738)
    SDS-PAGE - Recombinant human KMT1E / SETDB1 protein (Active) (ab271738)

    SDS-PAGE analysis of 2 µg ab271738.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab271738 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab271738 は論文での使用が確認できていません。

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