Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: CellDiff, Functional Studies, SDS-PAGE
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
製品名Recombinant human KGF/FGF-7 protein
KGF/FGF-7 タンパク質・ペプチド 製品一覧
Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors using Cell Counting Kit-8 (CCK-8).
精製度> 98 % SDS-PAGE.
Endotoxin level is less than 0.1 ng per g (1EU/g).
タンパク質長Full length protein
Our Abpromise guarantee covers the use of ab9601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
備考The ED50, determined by the dose-dependent stimulation of of thymidine uptake by KGF-responsive BaF3 cells, is < 10.0 ng/ml, corresponding to a specific activity of > 1.0 x 105 units/mg.
Concentration information loading...
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
再構成For lot specific reconstitution information please contact our Scientific Support Team.
- FGF 7
機能Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
配列類似性Belongs to the heparin-binding growth factors family.
- Information by UniProt
Definitive endoderm derived from culturing human embryonic stem cells (HUES4) with Inhibin beta a (ab50051) and Wnt3a (ab81484) was further differentiated to gut tube endoderm as described in Kroon et al, Nat Biotechnol. (2008). KGF/FGF-7 (ab9601) was added to the media to direct differentiation towards gut tube endoderm. This image was taken 3 days after addition of KGF/FGF-7, where the gut tube endoderm marker TCF2 (ab59118 in green) is present. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab9601 は 1 報の論文で使用されています。
- Lybrand DB et al. Destruction complex dynamics: Wnt/ß-catenin signaling alters Axin-GSK3ß interactions in vivo. Development 146:N/A (2019). PubMed: 31189665