Recombinant human KDM6A / UTX protein (Active) (ab271785)

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Functional Studies - Recombinant human KDM6A / UTX protein (Active) (ab271785)
  • SDS-PAGE - Recombinant human KDM6A / UTX protein (Active) (ab271785)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: >= 58% SDS-PAGE
  • Active: Yes
  • Tags: DDDDK tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

製品の詳細

  • 製品名

    Recombinant human KDM6A / UTX protein (Active)
    KDM6A / UTX タンパク質・ペプチド 製品一覧

  • 生理活性

    Specific Activity: 0.096 pmol/min/μg.
     
    Assay Conditions: 10 µl reaction mix containing assay buffer with 50 mM HEPES (pH 7.4), 1 mM TCEP, 100 µM alpha-ketoglutarate, 25 µM iron, 0.1 mM ascorbic acid, 0.02% Tween-20, 0.5 µM biotinylated peptide substrate, and ab271785 (10 – 50ng) added to the wells. Add antibody against di-methylated K27 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.

  • 精製度

    >= 58 % SDS-PAGE.

  • 発現系

    Baculovirus infected Sf9 cells

  • アクセッション番号

    O15550

  • タンパク質長

    Protein fragment

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      MKSCGVSLATAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALG GLDSRLFGFV RFHEDGARTKALLGKAVRCYESLILKAEGKVESDFFCQ LGHFNLLLEDYPKALSAYQRYY SLQSDYWKNAAFLYGLGLVYFHYNAF QWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVN TDYESSLKHFQLAL VDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQ VK ATVLQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGR CYSSIGKV QDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQA YICAVQLDHGHAAAWMDLGT LYESCNQPQDAIKCYLNATRSKSCSNTS ALAARIKYLQAQLCNLPQGSLQNKTKLLPSIE EAWSLPIPAELTSRQG AMNTAQQNTSDNWSGGHAVSHPPVQQQAHSWCLTPQKLQHLEQL RANR NNLNPAQKLMLEQLESQFVLMQQHQMRPTGVAQVRSTGIPNGPTADSSLP TNSVSG QQPQLALTRVPSVSQPGVRPACPGQPLANGPFSAGHVPCSTS RTLGSTDTILIGNNHITG SGSNGNVPYLQRNALTLPHNRTNLTSSAEE PWKNQLSNSTQGLHKGQSSHSAGPNGERPL SSTGPSQHLQAAGSGIQN QNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKP SGNILT VPETSRHTGETPNSTASVEGLPNHVHQMTADAVCSPSHGDSKSPGLLSSD NPQL SALLMGKANNNVGTGTCDKVNNIHPAVHTKTDNSVASSPSSAIS TATPSPKSTEQTTTNS VTSLNSPHSGLHTINGEGMEESQSPMKTDLLL VNHKPSPQIIPSMSVSIYPSSAEVLKAC RNLGKNGLSNSSILLDKCPP PRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFC TNPNNPVT VIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTK KI WHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSEST SSDNSGRRRKGPFK TIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVS AGNLLSHVGHTILGMNTVQLYMKVPG SRTPGHQENNNFCSVNINIGPG DCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDL YEANVPVYRF IQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNK LQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLRE ALIAAGKEIIWH GRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIV HCQDCARKTSGNLENFVVLEQYKM EDLMQVYDQFTLAPPLPSASS

    • 分子量情報

      155 by SDS-PAGE

    • 領域

      1 to 1401

    • タグ

      DDDDK tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab271785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態

    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.01% TCEP

    80 µg/ml flag peptide

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名

    • bA386N14.2
    • bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)
    • bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX
    • DKFZp686A03225
    • Histone demethylase UTX
    • KABUK2
    • Kdm6a
    • KDM6A_HUMAN
    • Lysine (K) specific demethylase 6A
    • Lysine K specific demethylase 6A
    • Lysine specific demethylase 6A ubiquitously transcribed tetratricopeptide repeat gene X chromosome
    • Lysine-specific demethylase 6A
    • MGC141941
    • Ubiquitously transcribed tetratricopeptide repeat X chromosome
    • Ubiquitously transcribed tetratricopeptide repeat protein X linked
    • Ubiquitously transcribed TPR gene on the X chromosome
    • Ubiquitously transcribed TPR protein on the X chromosome
    • Ubiquitously transcribed X chromosome tetratricopeptide repeat protein
    • Ubiquitously-transcribed TPR protein on the X chromosome
    • Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein
    • UTX
    see all

  • 機能

    Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitent with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A.

  • 配列類似性

    Belongs to the UTX family.
    Contains 1 JmjC domain.
    Contains 8 TPR repeats.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

画像

  • Functional Studies - Recombinant human KDM6A / UTX protein (Active) (ab271785)
    Functional Studies - Recombinant human KDM6A / UTX protein (Active) (ab271785)

    Specific activity of ab271785 was 0.096 pmol/min/μg.

  • SDS-PAGE - Recombinant human KDM6A / UTX protein (Active) (ab271785)
    SDS-PAGE - Recombinant human KDM6A / UTX protein (Active) (ab271785)

    SDS-PAGE analysis of 1.2 µg ab271785.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab271785 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab271785 は論文での使用が確認できていません。

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