Recombinant human KDM2A protein (Active) (ab271507)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, Avi tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
-
製品名
Recombinant human KDM2A protein (Active)
KDM2A タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: 0.01 pmole/min/μg. -
精製度
>= 80 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
EPEEERIRYSQRLRGTMRRRYEDDGISDDEIEGKRTFDLEEKLHTNKYNA NFVTFMEGKDFNVEYIQRGGLRDPLIFKNSDGLGIKMPDPDFTVNDVKMC VGSRRMVDVMDVNTQKGIEMTMAQWTRYYETPEEEREKLYNVISLEFSHT RLENMVQRPSTVDFIDWVDNMWPRHLKESQTESTNAILEMQYPKVQKYCL MSVRGCYTDFHVDFGGTSVWYHIHQGGKVFWLIPPTAHNLELYENWLLSG KQGDIFLGDRVSDCQRIELKQGYTFVIPSGWIHAVYTPTDTLVFGGNFLH SFNIPMQLKIYNIEDRTRVPNKFRYPFYYEMCWYVLERYVYCITNRSHLT KEFQKESLSMDLELNGLESGNGDEEAVDREPRRLSSRRSVLTSPVANGVN LDYDGLGKTCRSLPSLKKTLAGDSSSDCSRGSHNGQVWDPQCAPRKDRQV HLTHFELEGLRCLVDKLESLPLHKKCVPTGIEDEDALIADVKILLEELAN SDPKLALTGVPIVQWPKRDKLKFPTRPKVRVPTIPITKPHTMKPAPRLTP VRPAAASPIVSGARRRRVRCRKCKACVQGECGVCHYCRDMKKFGGPGRMK QSCVLRQCLAPRLPHSVTCSLCGEVDQNEETQDFEKKLMECCICNEIVHP GCLQMDGEGLLNEELPNCWECPKCYQEDSSEKAQKRKMEESDEEAVQAK -
予測される分子量
82 kDa -
領域
2 to 700 -
タグ
DDDDK tag N-Terminus , Avi tag N-Terminus
-
特性
Our Abpromise guarantee covers the use of ab271507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
-
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
80 µg/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- [Histone-H3]-lysine-36 demethylase 1A
- CXXC-type zinc finger protein 8
- CXXC8
see all -
機能
Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. -
組織特異性
Widely expressed, with highest levels in brain, testis and ovary, followed by lung. -
配列類似性
Belongs to the JHDM1 histone demethylase family.
Contains 1 CXXC-type zinc finger.
Contains 1 F-box domain.
Contains 1 JmjC domain.
Contains 6 LRR (leucine-rich) repeats.
Contains 1 PHD-type zinc finger. -
ドメイン
The JmjC domain mediates demethylation activity and is required for satellite silencing.
The CXXC zinc finger preferentially recognizes nonmethylated CpG DNA, and binding is blocked when the CpG DNA is methylated. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleoplasm. Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab271507 は論文での使用が確認できていません。