Recombinant Human KDM1/LSD1 protein (ab132186)
Key features and details
- Expression system: Wheat germ
- Purity: >= 80% Purified via GST Tag
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE, ELISA
製品の詳細
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製品名
Recombinant Human KDM1/LSD1 protein
KDM1/LSD1 タンパク質・ペプチド 製品一覧 -
精製度
>= 80 % Purified via GST Tag.
Glutathione Sepharose -
発現系
Wheat germ -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MLSGKKAAAAAAAAAAAATGTEAGPGTAGGSENGSEVAAQPAGLSGPAEV GPGAVGERTPRKKEPPRASPPGGLAEPQGSAGPQAGPTVVPGSATPMETG IAETPEGRRTSRRKRAKVEYREMDESLANLSEDEYYSEEERNAKAEKEKK LPPPPPQAPPEEENESEPEEPSGQAGGLQDDSSGGYGDGQASGVEGAAFQ SRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLT FEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGK VIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLG AMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQADTVKVPKEKDE MVEQEFNRLLEATSYLSHQLDFNVLNNKPVSLGQALEVVIQLQEKHVKDE QIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPPRDITA EFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLSSRDR QILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPV ALAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLP LGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVN LFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGR CLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLM AQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIA DQFLGAMYTLPRQATPGVPAQQSPSM -
予測される分子量
122 kDa including tags -
領域
1 to 876 -
タグ
GST tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab132186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
SDS-PAGE
ELISA
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
関連情報
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別名
- Amine oxidase (flavin containing) domain 2
- Amine oxidase, flavin containing, 2
- AOF2
see all -
機能
Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the flavin monoamine oxidase family.
Contains 1 SWIRM domain. -
ドメイン
The SWIRM domain may act as an anchor site for a histone tail. -
細胞内局在
Nucleus. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab132186 は論文での使用が確認できていません。