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Recombinant Human KAT9 / Elp3 protein (ab105858)

Submit a review Q&A (1)
SDS-PAGE - Recombinant Human KAT9 / Elp3 protein (ab105858)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 75% Densitometry
    • Suitable for: WB

    製品の詳細

    • 製品名

      Recombinant Human KAT9 / Elp3 protein

    • 精製度

      > 75 % Densitometry.
      ab105858 was determined to be >75% pure by densitometry. Affinity purified.

    • 発現系

      Baculovirus infected Sf9 cells

    • アクセッション番号

      Q9H9T3

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 予測される分子量

        86 kDa

      • 領域

        1 to 547

    特性

    Our Abpromise guarantee covers the use of ab105858 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Western blot

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride

    関連情報

    • 別名

      • DKFZp761L098
      • Elongation protein 3 homolog
      • Elongation protein 3 homolog (S. cerevisiae)
      • ELONGATION PROTEIN 3, S. CEREVISIAE, HOMOLOG OF
      • elongator acetyltransferase complex subunit 3
      • Elongator complex protein 3
      • elp3
      • ELP3_HUMAN
      • FLJ10422
      • hELP3
      • Hypothetical protein DKFZp761L098
      • Kat9
      see all

    • 機能

      Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. May also have a methyltransferase activity.

    • 配列類似性

      Belongs to the ELP3 family.
      Contains 1 N-acetyltransferase domain.

    • 細胞内局在

      Cytoplasm. Nucleus.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human KAT9 / Elp3 protein (ab105858)
      SDS-PAGE - Recombinant Human KAT9 / Elp3 protein (ab105858)
      ab105858 was determined to be >75% pure by densitometry.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab105858 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab105858 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Is this purified protein fragment enzymatically active

    Read More
    Answer

    The lab have confirmed that under our own assay conditions, SIRT4 (ab101713) and KAT9(ELP3) (ab105858) proteins have not shown significant activity and that is why we have not labeled them as active. However, some phosphorylation may be present as we do not test specifically for phosphorylation, we only test for activity. SIRT4 is expressed in E.Coli and KAT9 is expressed in Sf9 cells so KAT9 is more likely to have some post translational modifications.

    Read More

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