Recombinant human IRAK-1 protein (Active) (ab271553)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human IRAK-1 protein (Active)
IRAK-1 タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity ≥40 pmol/min/µg.
Assay was done in Kinase buffer containing 1 mM DTT using MBP substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min.
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精製度
>= 90 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
SVSLLQGARP FPFCWPLCEI SRGTHNFSEE LKIGEGGFGC VYRAVMRNTV YAVKRLKENA DLEWTAVKQS FLTEVEQLSR FRHPNIVDFA GYCAQNGFYC LVYGFLPNGS LEDRLHCQTQ ACPPLSWPQR LDILLGTARA IQFLHQDSPS LIHGDIKSSN VLLDERLTPK LGDFGLARFS RFAGSSPSQS SMVARTQTVR GTLAYLPEEY IKTGRLAVDT DTFSFGVVVL ETLAGQRAVK THGARTKYLK DLVEEEAEEA GVALRSTQST LQAGLAADAW AAPIAMQIYK KHLDPRPGPC PPELGLGLGQ LACCCLHRRA KRRPPMTQVY ERLEKLQAVV AGVPG -
予測される分子量
40 kDa including tags -
領域
186 to 530 -
タグ
His tag N-Terminus -
配列の追加情報
Genbank: NM_001569
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関連製品
特性
Our Abpromise guarantee covers the use of ab271553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 0.34% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- AA48924
- Il1rak
- Interleukin 1 receptor associated kinase 1
see all -
機能
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. -
組織特異性
Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. - Information by UniProt
画像
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Functional Studies - Recombinant Human IRAK-1 protein (Tagged) (ab271553)
Functional analysis of ab271553.
Specific activity ≥40 pmol/min/µg.
Assay was done in Kinase buffer containing 1 mM DTT using MBP substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min.
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SDS-PAGE - Recombinant Human IRAK-1 protein (Tagged) (ab271553)SDS-PAGE analysis of ab271553.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (0)
ab271553 は論文での使用が確認できていません。
