Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品名Recombinant human IRAK-1 protein (Active)
IRAK-1 タンパク質・ペプチド 製品一覧
Specific activity ≥40 pmol/min/µg.
Assay was done in Kinase buffer containing 1 mM DTT using MBP substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min.
精製度>= 90 % SDS-PAGE.
発現系Baculovirus infected Sf9 cells
配列SVSLLQGARP FPFCWPLCEI SRGTHNFSEE LKIGEGGFGC VYRAVMRNTV YAVKRLKENA DLEWTAVKQS FLTEVEQLSR FRHPNIVDFA GYCAQNGFYC LVYGFLPNGS LEDRLHCQTQ ACPPLSWPQR LDILLGTARA IQFLHQDSPS LIHGDIKSSN VLLDERLTPK LGDFGLARFS RFAGSSPSQS SMVARTQTVR GTLAYLPEEY IKTGRLAVDT DTFSFGVVVL ETLAGQRAVK THGARTKYLK DLVEEEAEEA GVALRSTQST LQAGLAADAW AAPIAMQIYK KHLDPRPGPC PPELGLGLGQ LACCCLHRRA KRRPPMTQVY ERLEKLQAVV AGVPG
予測される分子量40 kDa including tags
領域186 to 530
タグHis tag N-Terminus
Our Abpromise guarantee covers the use of ab271553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 0.34% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Interleukin 1 receptor associated kinase 1
機能Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
組織特異性Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain.
翻訳後修飾Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.