Recombinant human IL-7 protein (ab9629)

Submit a review Q&A (1)References (1)

製品の詳細

  • 製品名

    Recombinant human IL-7 protein
    IL-7 タンパク質・ペプチド 製品一覧

  • 生理活性

    Determined by its ability to stimulate the proliferation of mouse 2E8 cells. The expected-ED50-is ≤ 0.5 ng/mL, corresponding to a specific activity of ≥ 2 x 106-units/mg.

  • 精製度

    >= 98 % SDS-PAGE.
    Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses.

  • エンドトキシン・レベル

    = 1.000 Eu/µg

  • 発現系

    Escherichia coli

  • アクセッション番号

    P13232

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH

    • 予測される分子量

      18 kDa

    • 領域

      26 to 177

    • 配列の追加情報

      The aa sequence corresponds to the mature full length protein without the signal peptide from aa 1-25.

関連製品

特性

Our Abpromise guarantee covers the use of ab9629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

  • 製品の状態

    Lyophilized
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Constituent: 0.06005% Acetic acid

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Centrifuge vial prior to opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex. Store at 2°C to 8°C for 1 week, or prepare for extended storage. Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA). Store working aliquots at -20°C to -80°C.

関連情報

  • 別名

    • IL 7
    • IL-7
    • Il7
    • IL7_HUMAN
    • Interleukin 7
    • Interleukin-7
    • LP-1
    • Lymphopoietin 1
    see all

  • 機能

    Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.

  • 配列類似性

    Belongs to the IL-7/IL-9 family.

  • 細胞内局在

    Secreted.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab9629 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9629 は 1 報の論文で使用されています。

  • Kelly EA  et al. Potential contribution of IL-7 to allergen-induced eosinophilic airway inflammation in asthma. J Immunol 182:1404-10 (2009). Functional Studies . PubMed: 19155487

レビューと Q&A

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Question

What should the protein be reconstituted with?

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Answer

Thank you for your enquiry. For reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Note: This solution is slow to dissolve. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. Please let me know if you have any additional questions.

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