Recombinant human IL-17RA Receptor protein (Active) (Biotin) (ab271551)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: Avi tag C-Terminus, Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human IL-17RA Receptor protein (Active) (Biotin)
IL-17RA Receptor タンパク質・ペプチド 製品一覧 -
生理活性
Active in a IL-17RA:IL-17A binding assay.
100 ng of IL-17A was coated onto a 96-well plate overnight. The indicated amount of ab271551 was added in IL-17 Assay Buffer and allowed to incubate at room temperature for 2 hr. Strep-HRP was added, followed by chemiluminescent substrate and detection.
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精製度
>= 80 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
LRLLDHRALV CSQPGLNCTV KNSTCLDDSW IHPRNLTPSS PKDLQIQLHF AHTQQGDLFP VAHIEWTLQT DASILYLEGA ELSVLQLNTN ERLCVRFEFL SKLRHHHRRW RFTFSHFVVD PDQEYEVTVH HLPKPIPDGD PNHQSKNFLV PDCEHARMKV TTPCMSSGSL WDPNITVETL EAHQLRVSFT LWNESTHYQI LLTSFPHMEN HSCFEHMHHI PAPRPEEFHQ RSNVTLTLRN LKGCCRHQVQ IQPFFSSCLN DCLRHSATVS CPEMPDTPEP IPDYMPLW -
予測される分子量
62 kDa including tags -
分子量情報
Protein runs at higher than predicted MW on SDS-PAGE due to glycosylation. -
領域
33 to 320 -
タグ
Avi tag C-Terminus , Fc tag C-Terminus -
配列の追加情報
Human IgG1 Fc.
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標識
Biotin
関連製品
特性
Our Abpromise guarantee covers the use of ab271551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
Enzymatically biotinylated using AviTagTM technology.
Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80°C.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- CANDF5
- CD217
- CD217 antigen
see all -
機能
Receptor for IL17A and IL17F. Binds its IL17A ligand with low affinity, suggesting that additional components are involved in IL17A-induced signaling. -
組織特異性
Widely expressed. -
関連疾患
Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5) [MIM:613953]. CANDF5 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. -
配列類似性
Contains 1 SEFIR domain. -
翻訳後修飾
Glycosylated. -
細胞内局在
Membrane. - Information by UniProt
画像
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Functional Studies - Recombinant Human IL-17RA Receptor protein (Tagged) (Biotin) (ab271551)
Functional analysis of ab271551.
Active in a IL-17RA:IL-17A binding assay.
100 ng of IL-17A was coated onto a 96-well plate overnight. The indicated amount of ab271551 was added in IL-17 Assay Buffer and allowed to incubate at room temperature for 2 hr. Strep-HRP was added, followed by chemiluminescent substrate and detection.
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SDS-PAGE - Recombinant Human IL-17RA Receptor protein (Tagged) (Biotin) (ab271551)SDS-PAGE analysis of ab271551.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271551 は論文での使用が確認できていません。