Recombinant human IDH2 protein (ab198092)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 59% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human IDH2 protein
IDH2 タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: 14,200 pmol/min/µg.
Assay Conditions:
The reaction mixture contained 50 mM Tris, pH 7.4, 5 mM MgCl2, 200 µM DL-isocitrate, 200 µM NADP+, and various amount of ab198092 in a volume of 200 µL. Reactions were initiated with the addition of ab198092, and the increase in NADPH absorbance at 340 nm was continuously monitored for 7 min. Molar extinction coefficient of NADPH is 6,220 M-1cm-1.
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精製度
>= 59 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MAGYLRVVRSLCRASGSRPAWAPAALTAPTSQEQPRRHYADKRIKVAKPV VEMDGDEMTRIIWQFIKEKLILPHVDIQLKYFDLGLPNRDQTDDQVTIDS ALATQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIRNILGGTVFRE PIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKD GSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMST KNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHRLIDDMVAQVLKS SGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGT VTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEK VCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALG RQDYKDDDDK -
予測される分子量
52 kDa including tags -
領域
1 to 452 -
タグ
DDDDK tag C-Terminus -
配列の追加情報
NM_002168.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab198092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Contains 90 µg/ml DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- D2HGA2
- ICD-M
- IDH
see all -
機能
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. -
関連疾患
D-2-hydroxyglutaric aciduria 2
Glioma
enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. -
配列類似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
翻訳後修飾
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3. -
細胞内局在
Mitochondrion. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab198092 は 1 報の論文で使用されています。
- Intlekofer AM et al. Acquired resistance to IDH inhibition through trans or cis dimer-interface mutations. Nature 559:125-129 (2018). PubMed: 29950729