アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    recombinant-human-idh1-protein-ab113858.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Energy Metabolism
Share by email

Recombinant human IDH1 protein (ab113858)

  • Datasheet
Submit a review Q&A (3)References (1)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

SDS-PAGE - Recombinant human IDH1 protein (ab113858)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    こちらの製品もご検討ください

    アッセイ
    Product image
    Isocitrate Dehydrogenase Assay Kit (Colorimetric) (ab102528)
    一次抗体
    Product image
    Anti-Zyxin (phospho S142) antibody (ab78910)
    タンパク質
    Product image
    Recombinant human Lactate Dehydrogenase protein (Active) (ab93699)

    関連製品

    製品の詳細

    • 製品名

      Recombinant human IDH1 protein
      IDH1 タンパク質・ペプチド 製品一覧
    • 生理活性

      Specific activity: > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C. Specific activity: > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C.

    • 精製度

      > 95 % SDS-PAGE.
      ab113858 was purified using conventional chromatography.
    • 発現系

      Escherichia coli
    • アクセッション番号

      O75874
    • タンパク質長

      Full length protein
    • Animal free

      No
    • 由来

      Recombinant
      • 生物種

        Human
      • 配列

        MGSSHHHHHHSSGLVPRGSHMSKKISGGSVVEMQGDEMTRIIWELIKEKL IFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDE KRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIII GRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAM GMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEI YDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDS VAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPI ASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACI KGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL
      • 予測される分子量

        49 kDa including tags
      • 領域

        1 to 414
      • タグ

        His tag N-Terminus

    関連製品

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-IDH1 antibody (ab94571)

    特性

    Our Abpromise guarantee covers the use of ab113858 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Mass Spectrometry

      SDS-PAGE

    • 質量分析

      MALDI-TOF
    • 製品の状態

      Liquid
    • 備考

       This product was previously labelled as Isocitrate dehydrogenase

       

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.002% PMSF, 0.02% DTT, 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    関連情報

    • 別名

      • Cytosolic NADP isocitrate dehydrogenase
      • Cytosolic NADP-isocitrate dehydrogenase
      • Epididymis luminal protein 216
      • Epididymis secretory protein Li 26
      • HEL-216
      • HEL-S-26
      • ICDH
      • IDCD
      • IDH
      • IDH1
      • IDHC_HUMAN
      • IDP
      • IDPC
      • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic
      • Isocitrate dehydrogenase [NADP] cytoplasmic
      • Isocitrate dehydrogenase 1 (NADP+) soluble
      • NADP dependent isocitrate dehydrogenase cytosolic
      • NADP dependent isocitrate dehydrogenase peroxisomal
      • NADP(+)-specific ICDH
      • Oxalosuccinate decarboxylase
      • PICD
      see all
    • 関連疾患

      Glioma
      Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
    • 配列類似性

      Belongs to the isocitrate and isopropylmalate dehydrogenases family.
    • 翻訳後修飾

      Acetylation at Lys-374 dramatically reduces catalytic activity.
    • 細胞内局在

      Cytoplasm. Peroxisome.
    • Target information above from: UniProt accession O75874 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    画像

    • SDS-PAGE - Recombinant human IDH1 protein (ab113858)
      SDS-PAGE - Recombinant human IDH1 protein (ab113858)
      15% SDS-PAGE analysis of 3 µg ab113858.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (1)

    ab113858 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab113858 は 1 報の論文で使用されています。

    • Intlekofer AM  et al. L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH. Nat Chem Biol 13:494-500 (2017). PubMed: 28263965

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Question

    The gel is a regular SDS-PAGE (10%), the sample was prepared in a standard fashion (1x SDS loading buffer with BME, boiled for 10 min). The sample has been at -20C since arriving. There are no degradation products on the gel, as would be the case if storage or transportation was an issue. The two other bands are higher MW contaminants. The isocitrate DHase does appear to be active. Gel image is attached (first lane marker, second lane 1uL of sample, third lane 2uL of sample). Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on May 16 2012

    Answer

    Thank you for your reply.

    I was in touch with the supplying lab and they weren't sure why the higher band was being detected.They verified this protein with 15% SDS-PAGE and hadn't identified anyimpurities above 70kDa.
    They wondered ifthis higher MW band maybe at approximately 97kDa? If so, it could correspond to its homodimer form. Can you identify the MW marker sizes?
    If that's not the case, would you like a replacement protein? How would you like to proceed?

    Read More

    Abcam Scientific Support

    Answered on May 16 2012

    Question

    Hello: We recently purchased the product listed above (recom isocitrate dehydrogenase) and the protein is only about 50% pure. We are usually extremely happy with both your products and customer service (you very nicely solved a problem we had with the also purchased IDH assay kit) but I feel that 50% purity is a little on the low side. I certainly would not expect 100% and even the >95% quoted on the website would be tough to obtain. See attached gel, the lower band is IDH at correct MW (these are not degradation products, single contaminant band at a higher MW on a denaturing gel). Is there anyway to get higher purity on this protein?

    Read More

    Abcam community

    Verified customer

    Asked on May 15 2012

    Answer

    Thank you for your inquiry. I am sorry to hear about the purity problem with ab113858.

    Do you think you could resend the gel image? Unfortunately it didn't go through. Usually .jpg, .ppt, .doc, or .pdf transfer well.

    Can you tell me more about the gel conditions?

    How was the protein stored?

    Thank you for sending this extra information. I will have to go back to the supplying laboratory and ask for their input since this is the first we've heard about purity concerns with this product and I want to make sure we have as many details. I look forward to your reply.

    Read More

    Abcam Scientific Support

    Answered on May 15 2012

    Question

    Inquiry: Is this product (Isocitrate dehydrogenase protein (ab113858)) active? There is another IDH product you have that specifically states "active" but it is the yeast version of the protein, and I would like the human. I would assume that the product is an active enzyme but since the other product explicitly states "active" I wanted to make sure.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 19 2012

    Answer

    Thank you for contacting Abcam.

    The bioactivity has just been tested and the information os below:

    Specific activity: > 0.7 units/ml. One unit will convert 1.0 umole of isocitrate to alpha-ketoglutarate per minute at pH7.5 at 25C.

    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    Answered on Apr 19 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    • ターゲット別に見る
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2022 Abcam plc. All rights reserved.