Recombinant human IDH1 (mutated R132H) protein (ab198123)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human IDH1 (mutated R132H) protein
IDH1 タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: 53 pmol/min/μg.
Assay conditions: ab198123 reductive activity was measured in 200 μl reaction containing 25 mM Tris (pH 7.4), 150 mM NaCl, 10 mM MgCl2, 0.03% BSA, 1 mM -Ketoglutarate, 10 μM NADPH and IDH. Depletion of NADPH was monitored over time at 340 nm.
Depletion of NADPH was monitored continuously at Abs340 nm for 20 min. Molar extinction coefficient of NADPH is 6,200
M-1cm-1. -
精製度
> 90 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRD ATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIR NILGGTVFREAIICKNIPRLVSGWVKPIIIGHHAYGDQYRATDFVVPGPG KVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMA LSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRL IDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDG KTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNK ELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDK LGENLKIKLAQAKLDYKDDDDK -
予測される分子量
48 kDa including tags -
領域
1 to 414 -
修飾
mutated R132H -
タグ
DDDDK tag C-Terminus -
配列の追加情報
GenBank Accession No. NM_005896
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab198123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
This product was previously labelled as Isocitrate dehydrogenase
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.02% Potassium chloride
Also contains 80 µg/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Cytosolic NADP isocitrate dehydrogenase
- Cytosolic NADP-isocitrate dehydrogenase
- Epididymis luminal protein 216
see all -
関連疾患
Glioma
Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. -
配列類似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
翻訳後修飾
Acetylation at Lys-374 dramatically reduces catalytic activity. -
細胞内局在
Cytoplasm. Peroxisome. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab198123 は論文での使用が確認できていません。