Recombinant human HGF protein (Active) (ab632)

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Sandwich ELISA - Recombinant human HGF protein (ab632)

    Key features and details

    • Expression system: BTI-TN-5B1-4 cells
    • Purity: >= 98% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: HPLC, Functional Studies, SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant human HGF protein (Active)
      HGF タンパク質・ペプチド 製品一覧

    • 生理活性

      The ED50, as determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells, was found to be in the range of 20.0-40.0 ng/ml. 

    • 精製度

      >= 98 % SDS-PAGE.
      >=98% HPLC analyses. Sterile filtered.

    • エンドトキシン・レベル

      < 1.000 Eu/µg

    • 発現系

      BTI-TN-5B1-4 cells

    • アクセッション番号

      P14210

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLRVVNGIPT RTNIGWMVSL RYRNKHICGG SLIKESWVLT ARQCFPSRDL KDYEAWLGIH DVHGRGDEKC KQVLNVSQLV YGPEGSDLVL MKLARPAVLD DFVSTIDLPN YGCTIPEKTS CSVYGWGYTG LINYDGLLRV AHLYIMGNEK CSQHHRGKVT LNESEICAGA EKIGSGPCEG DYGGPLVCEQ HKMRMVLGVI VPGRGCAIPN RPGIFVRVAY YAKWIHKIIL TYKVPQS

      • 予測される分子量

        80 kDa

      • 領域

        32 to 728

      • 配列の追加情報

        α-chain and β-chain, held together by a single disulfide bond, resulting in formation of a biologically active heterodimer. Full length mature protein, without the signal peptide.

    関連製品

      特性

      Our Abpromise guarantee covers the use of ab632 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • アプリケーション

        HPLC

        Functional Studies

        SDS-PAGE

      • 製品の状態

        Lyophilized

      • 備考

        Expression System = Baculovirus infected High-5 cells.

      • Concentration information loading...

      前処理および保存

      • 保存方法および安定性

        Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

        Constituents: 1.16% Sodium chloride, 0.12% Tris, 1.74% DL-Arginine

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • 再構成
        Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

      関連情報

      • 別名

        • DFNB39
        • F TCF
        • Fibroblast derived tumor cytotoxic factor
        • Hepatocyte growth factor
        • Hepatocyte growth factor (hepapoietin A; scatter factor)
        • Hepatocyte growth factor beta chain
        • Hepatocyte growth factor precursor
        • Hepatopoietin A
        • Hepatopoietin-A
        • Hgf
        • HGF_HUMAN
        • HGFB
        • HPTA
        • Lung fibroblast derived mitogen
        • OTTHUMP00000161349
        • OTTHUMP00000206710
        • OTTHUMP00000206711
        • OTTHUMP00000206712
        • OTTHUMP00000206713
        • OTTHUMP00000206730
        • Scatter factor
        • SF
        see all

      • 機能

        Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.

      • 関連疾患

        Deafness, autosomal recessive, 39

      • 配列類似性

        Belongs to the peptidase S1 family. Plasminogen subfamily.
        Contains 4 kringle domains.
        Contains 1 PAN domain.
        Contains 1 peptidase S1 domain.
      • Information by UniProt

      画像

      • Sandwich ELISA - Recombinant human HGF protein (ab632)
        Sandwich ELISA - Recombinant human HGF protein (ab632)
        Standard curve for HGF (Analyte: ab632); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab10829 at 5µg/ml and Detector Antibody ab24865 at 0.5µg/ml.

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab632 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab632 は論文での使用が確認できていません。

      レビューと Q&A

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      レビューを送る 質問を送る

      Question

      Is the protein HGF ab632 and ab4261 supplied as the active form?

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      Answer

      I can confirm the product ab632 human HGF is supplied as the active (mature form). Please contact us again if you need further information,

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