Recombinant human HDAC9 protein (ab80350)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human HDAC9 protein
HDAC9 タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: >1000 U/ug. One U =1pmol/min. Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 uM BPS HDAC substrate, and 0.2 ng/ul HDAC9. Incubation condition: 30 min at 37ºC.
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精製度
> 95 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
領域
604 to 1066
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab80350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.8004% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- HD 7
- HD 7B
- HD 9
see all -
機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter. -
組織特異性
Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level). -
関連疾患
Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein. -
配列類似性
Belongs to the histone deacetylase family. HD type 2 subfamily. -
翻訳後修飾
Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Sumoylated. -
細胞内局在
Nucleus. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab80350 は論文での使用が確認できていません。