Recombinant human HDAC6 protein (Active) (ab42632)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 65% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Inhibition Assay, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human HDAC6 protein (Active)
HDAC6 タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: ≥350 pmol/min/µg.Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM BPS HDAC substrate 3, and HDAC6 (3.1 – 200 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature. -
精製度
> 65 % SDS-PAGE.
Protein was purified by affinity chromatography and gel filtration. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELG LEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAV LDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVT HPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYI AWPLQGWQATFGGGDH PPKSDPMGHHHHHHGRRRASVAAGILVPRGSP GLDGIYARGIQMTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGA VPRSIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLV LDEQLNEFHCLWDDSFPEGPERLHAIKEQLIQEGLLDRCVSFQARFAEKE ELMLVHSLEYIDLMETTQYMNEGELRVLADTYDSVYLHPNSYSCACLASG SVLRLVDAVLGAEIRNGMAIIRPPGHHAQHSLMDGYCMFNHVAVAARYAQ QKHRIRRVLIVDWDVHHGQGTQFTFDQDPSVLYFSIHRYEQGRFWPHLKA SNWSTTGFGQGQGYTINVPWNQVGMRDADYIAAFLHVLLPVALEFQPQLV LVAAGFDALQGDPKGEMAATPAGFAQLTHLLMGLAGGKLILSLEGGYNLR ALAEGVSASLHTLLGDPCPMLESPGAPCRSAQASVSCALEALEPFWEVLV RSTETVERDNMEEDNVEESEEEGPWEPPVLPILTWPVLQSRTGLVYDQNM MNHCNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTC HSAEYVGHLRATEKMKTRELHRESSNFDSIYICPSTFACAQLATGAACRL VEAVLSGEVLNGAAVVRPPGHHAEQDAACGFCFFNSVAVAARHAQTISGH ALRILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASS QIGRAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSA GFDAARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISE SMAACTRSLLGDPPPLLTLPRPPLSGALASITETIQVHRRYWRSLRVMKV EDREGPSSSKLVTKKAPQPAKPRLAERMTTREKKVLEAGMGKVTSASFGE ESTPGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTS EEAVGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGAT LAQTTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQG TTPQISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADS MLMQGSRGLTDQAIFYAVTPLPWCPHLVAVCPIPAAGLDVTQPCGDCGTI QENWVCLSCYQVYCGRYINGHMLQHHGNSGHPLVLSYIDLSAWCYYCQAY VHHQALLDVKNIAHQNKFGEDMPHPH" -
予測される分子量
161 kDa including tags -
領域
1 to 1215 -
タグ
GST tag N-Terminus -
配列の追加情報
BC069243. NP_006035 161 kDa including tags
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特性
Our Abpromise guarantee covers the use of ab42632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Inhibition Assay
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Constituents: 0.63% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.0165% Potassium chloride, 0.04% Tween, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- CPBHM
- FLJ16239
- HD 6
see all -
機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. -
配列類似性
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger. -
翻訳後修飾
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro. -
細胞内局在
Nucleus. Cytoplasm. It is mainly cytoplasmic, where it is associated with microtubules. - Information by UniProt
画像
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Specific Activity: ≥350 pmol/min/µg.Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 μM BPS HDAC substrate 3, and HDAC6 (3.1 – 200 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.
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4-20% SDS-PAGE - Recombinant human HDAC6 protein (Active) (ab42632).
Lane 1: 2µg HDAC6.
Lane 2: MW marker.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab42632 は 3 報の論文で使用されています。
- Huang Y et al. Discovery of Janus Kinase 2 (JAK2) and Histone Deacetylase (HDAC) Dual Inhibitors as a Novel Strategy for the Combinational Treatment of Leukemia and Invasive Fungal Infections. J Med Chem 61:6056-6074 (2018). PubMed: 29940115
- Zhang Y et al. Discovery of a fluorescent probe with HDAC6 selective inhibition. Eur J Med Chem 141:596-602 (2017). PubMed: 29102179
- Duan W et al. Design, synthesis, and antitumor evaluation of novel histone deacetylase inhibitors equipped with a phenylsulfonylfuroxan module as a nitric oxide donor. J Med Chem 58:4325-38 (2015). PubMed: 25906087