Recombinant Human Haptoglobin protein (Tagged-His Tag) (ab196135)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: HPLC, SDS-PAGE
製品の詳細
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製品名
Recombinant Human Haptoglobin protein (Tagged-His Tag)
Haptoglobin タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
Purity of ab196135 is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
VDSGNDVTDIADDGCPKPPEIAHGYVEHSVRYQCKNYYKLRTEGDGVYTL NDKKQWINKAVGDKLPECEADDGCPKPPEIAHGYVEHSVRYQCKNYYKLR TEGDGVYTLNNEKQWINKAVGDKLPECEAVCGKPKNPANPVQ ILGGHLDAKGSFPWQAKMVSHHNLTTGATLINEQWLLTTAKNLFLN HSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYSQVDIGLIKLKQKVS VNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVADQD QCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGS AFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKTIAENV DHHHHHH -
予測される分子量
44 kDa including tags -
分子量情報
Predicted MW: 15.9 and 28.3 kDa Apparent MW: 16 and 40-75 kDa (under reducing conditions) -
領域
19 to 406 -
タグ
His tag C-Terminus -
配列の追加情報
Amino acid sequence from 19-160 and 162-406. Amino acid 161 is missing from this product. 6-His tag is on the beta chain
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab196135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 99% PBS, 0.02% DTT
Supplied as a 0.2 µm filtered solution. -
再構成Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
関連情報
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別名
- Binding peptide
- BP
- Haptoglobin alpha chain
see all -
機能
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Anhaptoglobinemia -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab196135 は論文での使用が確認できていません。