製品の詳細

  • 製品名

    Recombinant human GSK3 beta protein (Active)
    GSK3 beta タンパク質・ペプチド 製品一覧
  • 生理活性

    148 nmol/min/mg

  • 精製度

    > 75 % Densitometry.

  • 発現系

    Baculovirus infected Sf9 cells
  • アクセッション番号

  • タンパク質長

    Full length protein
  • Animal free

    No
  • 由来

    Recombinant
    • 生物種

      Human
    • 予測される分子量

      73 kDa including tags
    • タグ

      GST tag N-Terminus
    • 配列の追加情報

      (Genbank Number NM_002093)

特性

Our Abpromise guarantee covers the use of ab60863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Functional Studies

    Western blot

  • 製品の状態

    Liquid
  • 備考

    ab204860 (Glycogen synthase 1 peptide) can be utilized as a substrate for assessing kinase activity.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride, 0.307% Glutathione

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名

    • Glycogen Synthase Kinase 3 Beta
    • Glycogen synthase kinase-3 beta
    • GSK 3 beta
    • GSK-3 beta
    • GSK3B
    • GSK3B_HUMAN
    • GSK3beta isoform
    • Serine/threonine-protein kinase GSK3B
    see all
  • 機能

    Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
  • 組織特異性

    Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
  • 配列類似性

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾

    Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
  • 細胞内局在

    Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt

画像

  • The specific activity of GSK3 beta (ab60863) was determined to be 218 nmol/min/mg as per activity assay protocol and was equivalent to 210 nmol/min/mg as per radiometric assay
  • SDS PAGE analysis of ab60863
  • SDS PAGE analysis of ab60863
  • Activity of ab60863: 148 nmol/min/mg (as per activity assay protocol).

  • The purity was determined to be >75% by densiometry.

    Approximate MW: 73 kDa.

  • ab60863 is a tagged recombinant protein with an expected molecular weight of 73kDa.

参考文献

This product has been referenced in:

  • Li L  et al. A TFEB nuclear export signal integrates amino acid supply and glucose availability. Nat Commun 9:2685 (2018). Read more (PubMed: 29992949) »
  • Suryo Rahmanto A  et al. FBW7 suppression leads to SOX9 stabilization and increased malignancy in medulloblastoma. EMBO J 35:2192-2212 (2016). Read more (PubMed: 27625374) »
See all 2 Publications for this product

レビューと Q&A

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Application
Functional Studies
Recombinant human GSK3ß (ab60863) was used to phosphorylate putative GSK3ß substrate (protein X). Evidence of phosphorylation was confirmed by:
(1) in-house generated phospho-specific antibody against the putative substrate (Protein X - T236 data shown)
(2) lambda phosphatase treatment of GSK3ß-treated protein X known phospho-specific protein interactor

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投稿 Dec 08 2014

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