Recombinant Human GDF8 / Myostatin protein (ab203723)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, WB, SDS-PAGE, MS
製品の詳細
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製品名
Recombinant Human GDF8 / Myostatin protein
GDF8 / Myostatin タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
Assessed by densitometry. Purifed using Ni-NTA chromatography. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
HHHHHHASNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLR LETAPNISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATT ETIITMPTESDFLMQVDGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVET PTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNW LKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSR RDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEF VFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIP AMVVDRCGCS -
予測される分子量
71 kDa including tags -
領域
24 to 375 -
タグ
His tag N-Terminus -
配列の追加情報
Mature protein and Propeptide without Signal peptide.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab203723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
Western blot
SDS-PAGE
Mass Spectrometry
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質量分析
LC-MS/MS -
製品の状態
Liquid -
備考
Frozen from a 0.25–0.5 mg/ml solution. Defrost at ambient temperature. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 20% Glycerol, 80% PBS
関連情報
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別名
- GDF 8
- GDF-8
- GDF8
see all -
機能
Acts specifically as a negative regulator of skeletal muscle growth. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
画像
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14 % SDS-PAGE analysis of ab203723:
1. Reduced and boiled sample, 2.5 μg/lane.
2. Non-reduced and non-boiled sample, 2.5 μg/lane.The approx. 50 kDa band represents the complex of Myostatin-Propeptide. The band corresponding to approx. 38 kDa represents cleaved form of Propeptide and similarly the approx. 12 kDa band belongs to cleaved Myostatin domain. Myostatin domains makes dimmers in non-reducing conditions resulting in bends of approx. 25 and 97 kDa, respectively.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab203723 は論文での使用が確認できていません。