Recombinant human Fyn protein (ab84696)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, WB, SDS-PAGE
製品の詳細
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製品名
Recombinant human Fyn protein
Fyn タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity: 166nmol/min/mg.
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精製度
> 90 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMGCV QCKDKEATKL TEERDGSLNQ SSGYRYGTDP TPQHYPSFGV TSIPNYNNFH AAGGQGLTVF GGVNSSSHTG TLRTRGGTGV TLFVALYDYE ARTEDDLSFH KGEKFQILNS SEGDWWEARS LTTGETGYIP SNYVAPVDSI QAEEWYFGKL GRKDAERQLL SFGNPRGTFL IRESETTKGA YSLSIRDWDD MKGDHVKHYK IRKLDNGGYY ITTRAQFETL QQLVQHYSER AAGLCCRLVV PCHKGMPRLT DLSVKTKDVW EIPRESLQLI KRLGNGQFGE VWMGTWNGNT KVAIKTLKPG TMSPESFLEE AQIMKKLKHD KLVQLYAVVS EEPIYIVTEY MNKGSLLDFL KDGEGRALKL PNLVDMAAQV AAGMAYIERM NYIHRDLRSA NILVGNGLIC KIADFGLARL IEDNEYTARQ GAKFPIKWTA PEAALYGRFT IKSDVWSFGI LLTELVTKGR VPYPGMNNRE VLEQVERGYR MPCPQDCPIS LHELMIHCWK KDPEERPTFE YLQSFLEDYF TATEPQYQPG ENL -
タグ
GST tag N-Terminus
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関連製品
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Related Products
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab84696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
Western blot
SDS-PAGE
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製品の状態
Liquid -
備考
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- C syn protooncogene
- Fyn
- FYN oncogene related to SRC FGR YES
see all -
機能
Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3. -
組織特異性
Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
細胞内局在
Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab84696 は論文での使用が確認できていません。