Recombinant Human Fibronectin type III EDB protein (ab209886)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant Human Fibronectin type III EDB protein
Fibronectin タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
ab209886 was sterile-filtered. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MASMTGGQQMGRGHHHHHHGNLYFQGGEFNVSVYTVKDDKESVPISDTII PEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFED FVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDL RFTNIGPDTMRVT -
予測される分子量
18 kDa including tags -
領域
1244 to 1377 -
タグ
His-T7 tag N-Terminus -
配列の追加情報
FN-III EDB domain constructed by fully synthetic gene synthesis with codon optimization and expressed with an N-terminal 29aa T7-His-TEV cleavage site.
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製品の詳細
Recombinant Human Fibronectin protein
関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab209886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
ab209886 was expressed in E.coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.
Extracellular matrix protein fibronectin (FN) plays an important role in cell adhesion. FN contains FN type I, II and III domains. The FN III repeat is generally about 90 amino acid long and to be composed of seven b-strands, forming two antiparallel b-sheets. In the C-terminal of human fibronectin region, there are 16 FN-III repeat domains. FN III domain derived proteins have been demonstrated to bind various proteins with high affinity. The alternatively-spliced extra-domain B (EDB) of fibronectin represents one of the best characterized markers of angiogenesis. This domain of 91-aminoacids is completely identical between mouse and man, is virtually absent in normal adult tissues (exception made for the endometrium in the proliferative phase and some vessels of the ovaries), but is strongly expressed in most aggressive solid cancer types, with a prominent vascular and/or stromal pattern of expression.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -80°C.
pH: 8.00
Constituent: 0.32% Tris HCl
Proprietary formulation of NaCl, KCl, EDTA, Arginine, DTT and Glycerol.
関連情報
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別名
- CIG
- Cold insoluble globulin
- Cold-insoluble globulin
see all -
機能
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
組織特異性
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
関連疾患
Glomerulopathy with fibronectin deposits 2 -
配列類似性
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
発生段階
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
翻訳後修飾
Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
細胞内局在
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab209886 は 1 報の論文で使用されています。
- Bayly-Jones C et al. Helical ultrastructure of the metalloprotease meprin α in complex with a small molecule inhibitor. Nat Commun 13:6178 (2022). PubMed: 36261433