Recombinant human FGF2 protein (Active) (ab9596)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: CellDiff, SDS-PAGE, Cellular Activation, HPLC
製品の詳細
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製品名
Recombinant human FGF2 protein (Active)
FGF2 タンパク質・ペプチド 製品一覧 -
生理活性
Determined by a cell proliferation assay using Balb/c 3T3 cells.
The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
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精製度
>= 95 % SDS-PAGE.
>= 95% HPLC Sterile filtered -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
AAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS -
予測される分子量
17 kDa -
領域
135 to 288
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特性
Our Abpromise guarantee covers the use of ab9596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Cell Differentiation
SDS-PAGE
Cellular Activation
HPLC
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.0605% Tris, 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Please reconstitute this product in 500ul 5mM Tris, pH7.6.
関連情報
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別名
- Basic fibroblast growth factor
- Basic fibroblast growth factor bFGF
- BFGF
see all -
機能
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107). -
組織特異性
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. -
配列類似性
Belongs to the heparin-binding growth factors family. -
翻訳後修飾
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. -
細胞内局在
Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. - Information by UniProt
画像
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Sandwich ELISA - Recombinant human FGF2 protein (ab9596)Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1ug/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5ug/ml.
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Cell differentiation - Recombinant human FGF2 protein (ab9596)This image is courtesy of Sun Yung, Centro de Investigación Príncipe Felipe.Human embryonic stem cells (H9) were differentiated both as embryoid bodies and monolayer cultures (on Matrigel) using a protocol described in Kennedy et al., 2007. BMP-4, FGF-basic (ab9596) and VEGF (ab9571) were sequentially added to the media to direct differentiation. These images were taken at day 8, where cell adhesion antigens CD31 (ab32457 in green) and CD34 (ab8536 in red) are abundant and represent primitive haematopoietic cells within the culture. Dapi staining is shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab9596 は 2 報の論文で使用されています。
- Song H et al. Effect of fibroblast growth factor 2 on degenerative endplate chondrocyte: From anabolism to catabolism. Exp Mol Pathol 118:104590 (2021). PubMed: 33285208
- Dias MH et al. Fibroblast Growth Factor 2 lethally sensitizes cancer cells to stress-targeted therapeutic inhibitors. Mol Oncol 13:290-306 (2019). PubMed: 30422399
