Bioactive grade

Recombinant Human Estrogen Receptor alpha protein (ab272167)

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Functional Studies - Recombinant Human Estrogen Receptor alpha protein (ab272167)
  • Western blot - Recombinant Human Estrogen Receptor alpha protein (ab272167)

Key features and details

  • Expression system: Yeast
  • Purity: > 70% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Suitable for: WB

製品の詳細

  • 製品名

    Recombinant Human Estrogen Receptor alpha protein
    Estrogen Receptor alpha タンパク質・ペプチド 製品一覧

  • 精製度

    > 70 % SDS-PAGE.

  • エンドトキシン・レベル

    < 1.000 Eu/µg

  • 発現系

    Yeast

  • アクセッション番号

    P03372

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPA VYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPL NSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFY RPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVW SCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGM MKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKR SKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLA DRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPG KLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKS IILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQ HQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLH APTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV

    • 予測される分子量

      66 kDa

    • 領域

      1 to 595

特性

Our Abpromise guarantee covers the use of ab272167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

  • 製品の状態

    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Store at -80°C.

    pH: 7.50
    Constituents: 1.21% Tris buffer, 0.6% Potassium chloride

    2 µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名

    • 7*/654 isoform
    • 7*/819 2 isoform
    • 7*/822 isoform
    • 8*/901 isoform
    • 8*/941 isoform
    • DKFZp686N23123
    • ER
    • ER alpha
    • ER-alpha
    • Era
    • ESR
    • ESR1
    • ESR1_HUMAN
    • ESRA
    • Estradiol receptor
    • Estrogen nuclear receptor alpha
    • Estrogen receptor
    • Estrogen receptor 1
    • Estrogen receptor alpha
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform
    • Estrogen receptor alpha delta 4 +49 isoform
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform
    • Estrogen receptor alpha E1 E2 1 2
    • Estrogen receptor alpha E1 N2 E2 1 2
    • ESTRR
    • NR3A1
    • Nuclear receptor subfamily 3 group A member 1
    see all

  • 機能

    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.

  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • ドメイン

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

  • 翻訳後修飾

    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).

  • 細胞内局在

    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt

画像

  • Functional Studies - Recombinant Human Estrogen Receptor alpha protein (ab272167)
    Functional Studies - Recombinant Human Estrogen Receptor alpha protein (ab272167)

    Performance of ab272167 activity (Red) compared with a major competitor (blue).

  • Western blot - Recombinant Human Estrogen Receptor alpha protein (ab272167)
    Western blot - Recombinant Human Estrogen Receptor alpha protein (ab272167)

    Western blot analysis profile of ab272167 (Lane 2) compared to a major competitor (Lane 1).

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab272167 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab272167 は論文での使用が確認できていません。

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