Recombinant human Eph receptor A4/SEK protein (Active) (ab271498)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Eph receptor A4/SEK protein (Active)
Eph receptor A4/SEK タンパク質・ペプチド 製品一覧 -
生理活性
Assay was done in kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 µM ATP. Reaction was performed at 30°C for 40 mins. Amount of ATP transferred was calculated using Kinase-Glo reagent.
Specific activity of 61 pmol/mon/µg.
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精製度
>= 90 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
REFAKEIDASCIKIEKVIGVGEFGEVCSGRLKVPGKREICVAIKTLKAGY TDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDA FLRKNDGRFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLV CKVSDFGMSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWS YGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPPPMDCPIALHQLMLD CWQKERSDRPKFGQIVNMLDKLIRNPNS -
予測される分子量
58 kDa -
領域
610 to 887 -
タグ
GST tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab271498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Cek 8
- CEK8
- EK8
see all -
機能
Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in a signal transduction process involved in hindbrain pattern formation. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
ドメイン
The protein kinase domain mediates interaction with NGEF/ephexin-1. -
細胞内局在
Membrane. - Information by UniProt
画像
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Functional Studies - Recombinant human Eph receptor A4/SEK protein (Active) (ab271498)
Specific activity of ab271498 is 61 pmol/min/µg.
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SDS-PAGE - Recombinant human Eph receptor A4/SEK protein (Active) (ab271498)SDS-PAGE analysis of 1.8 µg ab271498.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271498 は論文での使用が確認できていません。