Recombinant human Eph receptor A3 protein (ab84576)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Eph receptor A3 protein
Eph receptor A3 タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity of Eph receptor A3 was determined to be 102 nmol/min/mg as per activity assay protocol.
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精製度
> 90 % SDS-PAGE.
Purity was determined to be >90% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
領域
571 to 983
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関連製品
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab84576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- AW492086
- Cek4
- EC 2.7.10.1
see all -
機能
Receptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5. Could play a role in lymphoid function. -
組織特異性
Widely expressed. Highest level in placenta. -
関連疾患
Defects in EPHA3 may be a cause of colorectal cancer (CRC) [MIM:114500]. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
画像
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The specific activity of Eph receptor A3 (ab84576) was determined to be 19.8 nmol/min/mg as per activity assay protocol and was equivalent to 110 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab84576
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SDS PAGE analysis of ab84576
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Specific activity of Eph receptor A3 was determined to be 102 nmol/min/mg as per activity assay protocol.
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Purity of Eph receptor A3 was determined to be >90% by densitometry, approx. MW 72kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab84576 は論文での使用が確認できていません。