Recombinant human Elongin-B + Elongin C + VHL protein (Tagged) (ab271497)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
-
製品名
Recombinant human Elongin-B + Elongin C + VHL protein (Tagged) -
生理活性
Assay Conditions: Various amounts of ab271497 were incubated in 10-ul reaction with ARV-771 and GST-BRD3(BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
-
精製度
>= 90 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
アミノ酸配列 1
-
生物種
Human -
配列
DVFLMIRRHK TTIFTDAKES STVFELKRIV EGILKRPPDE QRLYKDDQLL DDGKTLGECG FTSQTARPQA PATVGLAFRA DDTFEALCIE PFSSPPELPD VMKPQDSGSS ANEQAVQ -
予測される分子量
14 kDa including tags -
領域
2 to 118 -
タグ
DDDDK tag N-Terminus -
配列の追加情報
GenBank: NM_007108
-
アミノ酸配列 2
-
生物種
Human -
配列
DGEEKTYGGC EGPDAMYVKL ISSDGHEFIV KREHALTSGT IKAMLSGPGQ FAENETNEVN FREIPSHVLS KVCMYFTYKV RYTNSSTEIP EFPIAPEIAL ELLMAANFLD C -
予測される分子量
13 kDa including tags -
領域
2 to 112 -
タグ
His tag N-Terminus -
配列の追加情報
GenBank: NM_005648
-
アミノ酸配列 3
-
生物種
Human -
配列
PRRAENWDEA EVGAEEAGVE EYGPEEDGGE ESGAEESGPE ESGPEELGAE EEMEAGRPRP VLRSVNSREP SQVIFCNRSP RVVLPVWLNF DGEPQPYPTL PPGTGRRIHS YRGHLWLFRD AGTHDGLLVN QTELFVPSLN VDGQPIFANI TLPVYTLKER CLQVVRSLVK PENYRRLDIV RSLYEDLEDH PNVQKDLERL TQERIAHQRM GD -
予測される分子量
25 kDa including tags -
領域
2 to 212 -
タグ
DDDDK tag N-Terminus -
配列の追加情報
GenBank: NM_000551
-
-
製品の詳細
Recombinant human Von Hippel Lindau/VHL + TCEB2/Elongin-B + Elongin-C protein (Tagged)
特性
Our Abpromise guarantee covers the use of ab271497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Functional Studies
-
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
細胞内局在
Von Hippel Lindau/VHL: Cytoplasm. Membrane. Nucleus. Found predominantly in the cytoplasm and with less amounts nuclear or membrane-associated and Cytoplasm. Nucleus. Equally distributed between the nucleus and the cytoplasm but not membrane-associated. TCEB2/Elongin-B: Nucleus. Elongin-C: Nucleus.
画像
-
Functional Studies - Recombinant human Von Hippel Lindau/VHL + TCEB2/Elongin-B + Elongin-C protein (Tagged) (ab271497)
ab271497-BRD3 interaction.
-
SDS-PAGE - Recombinant human Von Hippel Lindau/VHL + TCEB2/Elongin-B + Elongin-C protein (Tagged) (ab271497)SDS-PAGE analysis of ab271497.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271497 は論文での使用が確認できていません。
