Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 0.010 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品名Recombinant human DLL4 protein (Fc Chimera Active)
DLL4 タンパク質・ペプチド 製品一覧
Inhibits adipogenesis of 3T3L-1 cells and mesenchymal stem cells (MSCs). Induces the Notch target gene HES-1 when coated on a plate at 1µg/ml.
精製度>= 95 % SDS-PAGE.
Purified using affinity chromatography.
エンドトキシン・レベル< 0.010 Eu/µg
発現系HEK 293 cells
予測される分子量80 kDa including tags
領域1 to 529
タグFc tag C-Terminus
配列の追加情報Signal peptide and extracellular domain of human DLL4 (aa 1-529) are fused at the C-terminus to the Fc portion of human IgG1.
Our Abpromise guarantee covers the use of ab108557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab108557 interacts with Human Notch1 (as confirmed by Flow Cytometry).
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: PBS, 0.5% Trehalose
ab108557 is 0.2 µm filtered.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
再構成Reconstitute with 100µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions. Working aliquots are stable for up to 3 months when stored at -20°C.
- Delta 4
- delta 4 precursor
機能Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
組織特異性Expressed in vascular endothelium.
配列類似性Contains 1 DSL domain.
Contains 8 EGF-like domains.
ドメインThe Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
翻訳後修飾Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
- Information by UniProt
Interaction of Human Notch1 with Human DLL4.
HEK293 cells transfected with a Human Notch1 or a Human GITR ligand expressing vector were incubated with 25 μg/ml of Human GITR-Fc or ab108557. Cells were stained with anti-Human IgG (Fc specific) FITC conjugate for DLL4-Fc binding.
Induction of Hes-1 with the treatment of recombinant Human DLL4-Fc (ab108557).
A Mouse preadpipocyte cell line, 3T3L-1, was stimulated with 5 μg/ml of Human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-Mouse Hes1 or GAPDH.
Lane 1: hDLL4-Fc, 0 min.
Lane 2: hDLL4-Fc, 10 min.
Lane 3: hDLL4-Fc, 30 min.
Lane 4: hDLL4-Fc, 1 hr.
Lane 5: hDLL4-Fc, 2 hr.
Lane 6: hDLL4-Fc, 4 hr.
Lane 7: hDLL4-Fc, 8 hr.
Lane 8: hDLL4-Fc, 24 hr.
Adipogenesis inhibition of 3T3L-1 cells.
3T3L-1 cells (mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 cells, 3T3L-1 cells were cultured in adipogenic medium which was growth medium supplemented with 1 μM Dexamethasone, 0.5 mM IBMX, 10 μg/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-α (20 ng/ml) was added. Recombinant Human DLL4-Fc (ab108557) (5 μg/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 cells.
Adipogenesis inhibition of MSCs.
MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilinstreptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 μM Dexamethasone, 0.5mM IBMX, 10 μg/m lnsulin, 100 μM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-α (20 ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of ab108557 (5 μg/ml) or mCD137-Fc (5 μg/ml) in PBS for 2 hours at 37°C. Plates were then used to differentiate MSCs.
Adipogenesis inhibition of 3T3L-1 cells.
50 μg of cell lysates derived from hDLL4-Fc (ab108557) or non-treated 3T3L-1 cells, which had been either differentiated or undifferentiated, and were subjected to Western blot by using a Mouse adiponectin antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab108557 は 6 報の論文で使用されています。
- Bill M et al. EGFL7 Antagonizes NOTCH Signaling and Represents a Novel Therapeutic Target in Acute Myeloid Leukemia. Clin Cancer Res 26:669-678 (2020). PubMed: 31672772
- Kamalakar A et al. A non-canonical JAGGED1 signal to JAK2 mediates osteoblast commitment in cranial neural crest cells. Cell Signal 54:130-138 (2019). PubMed: 30529759
- Tiemeijer LA et al. Spatial patterning of the Notch ligand Dll4 controls endothelial sprouting in vitro. Sci Rep 8:6392 (2018). PubMed: 29686270
- Tang D et al. Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin ß1 in Keratinocyte Migration. Sci Rep 7:43926 (2017). PubMed: 28266574
- Faronato M et al. DMXL2 drives epithelial to mesenchymal transition in hormonal therapy resistant breast cancer through Notch hyper-activation. Oncotarget 6:22467-79 (2015). PubMed: 26093085
- Hale AT et al. Endothelial Kruppel-like factor 4 regulates angiogenesis and the Notch signaling pathway. J Biol Chem 289:12016-28 (2014). PubMed: 24599951