Recombinant human Cyclin T1 + Cdk9 protein (Active) (ab271464)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 66% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
-
製品名
Recombinant human Cyclin T1 + Cdk9 protein (Active) -
生理活性
26 pmol/min/μg
Assay Conditions: Assay was done in a Kinase buffer using PDKtide (0.1 mg/ml) as a substrate with 10 uM ATP at 30°C for 45 min. The amount of ATP transferred from autophosphorylation as well as substrate phosphorylation was calculated using Kinase-Glo reagent
-
精製度
>= 66 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
アミノ酸配列 1
-
生物種
Human -
配列
EGERKNNNKRWYFTREQLENSPSRRFGVDPDKELSYRQQAANLLQDMGQR LNVSQLTINTAIVYMHRFYMIQSFTQFPGNSVAPAALFLAAKVEEQPKKL EHVIKVAHTCLHPQESLPDTRSEAYLQQVQDLVILESIILQTLGFELTID HPHTHVVKCTQLVRASKDLAQTSYFMATNSLHLTTFSLQYTPPVVACVCI HLACKWSNWEIPVSTDGKHWWEYVDATVTLELLDELTHEFLQILEKTPNR LKRIWNWRACEAAKKTKADDRGTDEKTSEQTILNMISQSSSDTTIAGLMS MSTSTTSAVPSLPVSEESSSNLTSVEMLPGKRWLSSQPSFKLEPTQGHRT SENLALTGVDHSLPQDGSNAFISQKQNSKSVPSAKVSLKEYRAKHAEELA AQKRQLENMEANVKSQYAYAAQNLLSHHDSHSSVILKMPIEGSENPERPF LEKADKTALKMRIPVAGGDKAASSKPEEIKMRIKVHAAADKHNSVEDSVT KSREHKEKHKTHPSNHHHHHNHHSHKHSHSQLPVGTGNKRPGDPKHSSQT SNLAHKTYSLSSSFSSSSSTRKRGPSEETGGAVFDHPAKIAKSTKSSSLN FSFPSLPTMGQMPGHSSDTSGLSFSQPSCKTRVPHSKLDKGPTGANGHNT TQTIDYQDTVNMLHSLLSAQGVQPTQPTAFEFVRPYSDYLNPRSGGISSR SGNTDKPRPPPLPSEPPPPLPPLPK -
実際の分子量
107 kDa -
領域
2 to 726 -
タグ
GST tag N-Terminus
-
アミノ酸配列 2
-
生物種
Human -
配列
AKQYDSVECPFCDEVSKYEKLAKIGQGTFGEVFKARHRKTGQKVALKKVL MENEKEGFPITALREIKILQLLKHENVVNLIEICRTKASPYNRCKGSIYL VFDFCEHDLAGLLSNVLVKFTLSEIKRVMQMLLNGLYYIHRNKILHRDMK AANVLITRDGVLKLADFGLARAFSLAKNSQPNRYTNRVVTLWYRPPELLL GERDYGPPIDLWGAGCIMAEMWTRSPIMQGNTEQHQLALISQLCGSITPE VWPNVDNYELYEKLELVKGQKRKVKDRLKAYVRDPYALDLIDKLLVLDPA QRIDSDDALNHDFFWSDPMPSDLKGMLSTHLTSMFEYLAPPRRKGSQITQ QSTNQSRNPATTNQTEFERVF -
実際の分子量
69 kDa -
領域
2 to 372 -
タグ
GST tag N-Terminus
-
特性
Our Abpromise guarantee covers the use of ab271464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Functional Studies
-
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine), 0.05% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
細胞内局在
Cyclin T1: Nucleus. Cdk9: Nucleus.
画像
-
SDS-PAGE - Recombinant Human Cyclin T1 + Cdk9 protein (Tagged) (ab271464)SDS-PAGE analysis of ab271464.
-
Functional Studies - Recombinant Human Cyclin T1 + Cdk9 protein (Tagged) (ab271464)Specific activity of ab271464.
Assay Conditions: Assay was done in a Kinase buffer using PDKtide (0.1 mg/ml) as a substrate with 10 uM ATP at 30°C for 45 min. The amount of ATP transferred from autophosphorylation as well as substrate phosphorylation was calculated using Kinase-Glo reagent.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab271464 は論文での使用が確認できていません。