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Recombinant Human CXCL7/PBP protein (ab9556)

Submit a review Q&A (1)
Western blot - Recombinant Human CXCL7/PBP protein (ab9556)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 98% SDS-PAGE
    • Endotoxin level: < 0.100 Eu/µg
    • Suitable for: Functional Studies, SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Human CXCL7/PBP protein
      CXCL7/PBP タンパク質・ペプチド 製品一覧

    • 精製度

      > 98 % SDS-PAGE.
      Endotoxin level is less than 0.1 ng per g (1EU/g).

    • エンドトキシン・レベル

      < 0.100 Eu/µg

    • 発現系

      Escherichia coli

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        Recombinant Human NAP-2 is a 7.6 kDa protein containing 70 amino acid residues: AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KD GRKICLDP DAPRIKKIVQKKLAGDESAD

    特性

    Our Abpromise guarantee covers the use of ab9556 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Functional Studies

      SDS-PAGE

    • 製品の状態

      Lyophilized

    • 備考

      Previously labelled as CXCL7. 

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 再構成
      For lot specific reconstitution information please contact our Scientific Support Team.

    関連情報

    • 別名

      • B TG1
      • Beta TG
      • Beta thromboglobulin
      • Beta-TG
      • C-X-C motif chemokine 7
      • Chemokine (C X C motif) ligand 7
      • Connective tissue activating peptide III
      • CTAP 3
      • CTAP III
      • CTAP-III
      • CTAP-III(1-81)
      • CTAP3
      • CTAPIII
      • CXC chemokine ligand 7
      • CXCL 7
      • CXCL7
      • CXCL7_HUMAN
      • LA PF 4
      • LA-PF4
      • LDGF
      • Leukocyte derived growth factor
      • Leukocyte-derived growth factor
      • Low-affinity platelet factor IV
      • Macrophage-derived growth factor
      • MDGF
      • NAP 2
      • NAP-2
      • NAP-2(1-63)
      • NAP-2(1-66)
      • NAP-2(73)
      • NAP-2(74)
      • Neutrophil activating peptide 2
      • Neutrophil-activating peptide 2(1-63)
      • PBP
      • Platelet basic protein
      • PPBP
      • Pro platelet basic protein
      • Pro platelet basic protein (chemokine (C-X-C motif) ligand 7)
      • SCYB7
      • Small inducible cytokine subfamily B member 7
      • Small-inducible cytokine B7
      • TC1
      • TC2
      • TGB
      • TGB1
      • THBGB
      • THBGB1
      • Thrombocidin 1
      • Thrombocidin 2
      • Thromboglobulin, beta-1
      see all

    • 機能

      LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.

    • 配列類似性

      Belongs to the intercrine alpha (chemokine CxC) family.

    • 翻訳後修飾

      Proteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
      Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
      NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
      NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    画像

    • Western blot - Recombinant Human CXCL7/PBP protein (ab9556)
      Western blot - Recombinant Human CXCL7/PBP protein (ab9556)
      Recombinant Human CXCL7/PBP protein (ab9556) at 1/1000 dilution + Human CXCL7 active recombinant protein, 10 ng

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 11 kDa why is the actual band size different from the predicted?


      Exposure time: 5 seconds


      Blocking and Diluting buffer - 5% NFDM/TBST

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab9556 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9556 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Hi,

    I am looking for a neutralizing antibody for CXCL7, and I came across this product:Anti-NAP2 antibody (ab9554). I was wondering if this is indeed tested to show that is neutralizes CXCL7 and how much to use to block CXCL7. In addition, I was wondering if abcam sells the function CXCL7 protein.

    Also I am looking for a neutralizing antibody for Rantes and found Anti-Rantes antibody (ab9679).I was wondering if this is indeed tested to show that is neutralizes RANTES in solution and how much to use to block the protein. Also, if you could tell me if abcam sells the functional protein form of RANTES that would be great.

    Kind Regards,

    Read More
    Answer

    Thank you for your enquiry.

    For ab9554, Rabbit Anti Human NAP-2, this has been tested in Neutralisation as follows:
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human NAP-2 (100.0 ng/ml), a concentration of 5.0 - 10.0 ug/ml of this antibody was required. This may need some individual optimization.

    The recombinant protein used in this assay is NAP-2 protein ab9556.
    https://www.abcam.com/Recombinant-Human-CXCL7PBP-protein-ab9556.html (or use the following: https://www.abcam.com/Recombinant-Human-CXCL7PBP-protein-ab9556.html).

    For ab9679, Rabbit Anti Human RANTES, this has been tested in Neutralisation as follows:
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 0.4 – 0.6 μg/ml of this antibodywas required. This may need some individual optimization.

    The recombinant protein used in this assay, is Rantes protein ab9680.
    https://www.abcam.com/Recombinant-human-RANTES-protein-ab9680.html (or use the following: https://www.abcam.com/Recombinant-human-RANTES-protein-ab9680.html).

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact me.

    Read More

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