Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) (ab271467)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, DDDDK tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) -
生理活性
Cereblon-BRD3 interaction was assessed using A-screen technology. Various amounts of ab271467 were incubated in 10-ul reaction with dBET1 and GST-BRD3(BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
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精製度
>= 90 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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アミノ酸配列 1
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生物種
Human -
配列
AGEGDQQDAA HNMGNHLPLL PAESEEEDEM EVEDQDSKEA KKPNIINFDT SLPTSHTYLG ADMEEFHGRT LHDDDSCQVI PVLPQVMMIL IPGQTLPLQL FHPQEVSMVR NLIQKDRTFA VLAYSNVQER EAQFGTTAEI YAYREEQDFG IEIVKVKAIG RQRFKVLELR TQSDGIQQAK VQILPECVLP STMSAVQLES LNKCQIFPSK PVSREDQCSY KWWQKYQKRK FHCANLTSWP RWLYSLYDAE TLMDRIKKQL REWDENLKDD SLPSNPIDFS YRVAACLPID DVLRIQLLKI GSAIQRLRCE LDIMNKCTSL CCKQCQETEI TTKNEIFSLS LCGPMAAYVN PHGYVHETLT VYKACNLNLI GRPSTEHSWF PGYAWTVAQC KICASHIGWK FTATKKDMSP QKFWGLTRSA LLPTIPDTED EISPDKVILC L -
予測される分子量
51 kDa including tags -
領域
2 to 442 -
タグ
DDDDK tag N-Terminus -
配列の追加情報
GenBank: NM_016302
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アミノ酸配列 2
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生物種
Human -
配列
MSYNYVVTAQ KPTAVNGCVT GHFTSAEDLN LLIAKNTRLE IYVVTAEGLR PVKEVGMYGK IAVMELFRPK GESKDLLFIL TAKYNACILE YKQSGESIDI ITRAHGNVQD RIGRPSETGI IGIIDPECRM IGLRLYDGLF KVIPLDRDNK ELKAFNIRLE ELHVIDVKFL YGCQAPTICF VYQDPQGRHV KTYEVSLREK EFNKGPWKQE NVEAEASMVI AVPEPFGGAI IIGQESITYH NGDKYLAIAP PIIKQSTIVC HNRVDPNGSR YLLGDMEGRL FMLLLEKEEQ MDGTVTLKDL RVELLGETSI AECLTYLDNG VVFVGSRLGD SQLVKLNVDS NEQGSYVVAM ETFTNLGPIV DMCVVDLERQ GQGQLVTCSG AFKEGSLRII RNGIGIHEHA SIDLPGIKGL WPLRSDPNRE TDDTLVLSFV GQTRVLMLNG EEVEETELMG FVDDQQTFFC GNVAHQQLIQ ITSASVRLVS QEPKALVSEW KEPQAKNISV ASCNSSQVVV AVGRALYYLQ IHPQELRQIS HTEMEHEVAC LDITPLGDSN GLSPLCAIGL WTDISARILK LPSFELLHKE MLGGEIIPRS ILMTTFESSH YLLCALGDGA LFYFGLNIET GLLSDRKKVT LGTQPTVLRT FRSLSTTNVF ACSDRPTVIY SSNHKLVFSN VNLKEVNYMC PLNSDGYPDS LALANNSTLT IGTIDEIQKL HIRTVPLYES PRKICYQEVS QCFGVLSSRI EVQDTSGGTT ALRPSASTQA LSSSVSSSKL FSSSTAPHET SFGEEVEVHN LLIIDQHTFE VLHAHQFLQN EYALSLVSCK LGKDPNTYFI VGTAMVYPEE AEPKQGRIVV FQYSDGKLQT VAEKEVKGAV YSMVEFNGKL LASINSTVRL YEWTTEKELR TECNHYNNIM ALYLKTKGDF ILVGDLMRSV LLLAYKPMEG NFEEIARDFN PNWMSAVEIL DDDNFLGAEN AFNLFVCQKD SAATTDEERQ HLQEVGLFHL GEFVNVFCHG SLVMQNLGET STPTQGSVLF GTVNGMIGLV TSLSESWYNL LLDMQNRLNK VIKSVGKIEH SFWRSFHTER KTEPATGFID GDLIESFLDI SRPKMQEVVA NLQYDDGSGM KREATADDLI KVVEELTRIH -
予測される分子量
128 kDa including tags -
領域
1 to 1140 -
タグ
DDDDK tag N-Terminus -
配列の追加情報
GenBank: NM_001923
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アミノ酸配列 3
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生物種
Human -
配列
ADEAPRKGSF SALVGRTNGL TKPAALAAAP AKPGGAGGSK KLVIKNFRDR PRLPDNYTQD TWRKLHEAVR AVQSSTSIRY NLEELYQAVE NLCSHKVSPM LYKQLRQACE DHVQAQILPF REDSLDSVLF LKKINTCWQD HCRQMIMIRS IFLFLDRTYV LQNSTLPSIW DMGLELFRTH IISDKMVQSK TIDGILLLIE RERSGEAVDR SLLRSLLGML SDLQVYKDSF ELKFLEETNC LYAAEGQRLM QEREVPEYLN HVSKRLEEEG DRVITYLDHS TQKPLIACVE KQLLGEHLTA ILQKGLDHLL DENRVPDLAQ MYQLFSRVRG GQQALLQHWS EYIKTFGTAI VINPEKDKDM VQDLLDFKDK VDHVIEVCFQ KNERFVNLMK ESFETFINKR PNKPAELIAK HVDSKLRAGN KEATDEELER TLDKIMILFR FIHGKDVFEA FYKKDLAKRL LVGKSASVDA EKSMLSKLKH ECGAAFTSKL EGMFKDMELS KDIMVHFKQH MQNQSDSGPI DLTVNILTMG YWPTYTPMEV HLTPEMIKLQ EVFKAFYLGK HSGRKLQWQT TLGHAVLKAE FKEGKKEFQV SLFQTLVLLM FNEGDGFSFE EIKMATGIED SELRRTLQSL ACGKARVLIK SPKGKEVEDG DKFIFNGEFK HKLFRIKINQ IQMKETVEEQ VSTTERVFQD RQYQIDAAIV RIMKMRKTLG HNLLVSELYN QLKFPVKPGD LKKRIESLID RDYMERDKDN PNQYHYVA -
予測される分子量
88 kDa including tags -
領域
2 to 729 -
タグ
His tag N-Terminus -
配列の追加情報
Gen Bank: NM_003589
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アミノ酸配列 4
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生物種
Human -
配列
AAAMDVDTPS GTNSGAGKKR FEVKKWNAVA LWAWDIVVDN CAICRNHIMD LCIECQANQA SATSEECTVA WGVCNHAFHF HCISRWLKTR QVCPLDNREW EFQKYGH -
領域
2 to 108 -
タグ
His tag N-Terminus -
配列の追加情報
GenBank: NM_014248
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製品の詳細
Recombinant human DDB1 + Cullin 4A/CUL-4A + RBX1 + CRBN protein (Active)
特性
Our Abpromise guarantee covers the use of ab271467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
Co-expressed in HEK-293 cells.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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細胞内局在
DDB1: Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage. RBX1: Cytoplasm. Nucleus. CRBN: Cytoplasm. Nucleus. Membrane.
画像
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Functional Studies - Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) (ab271467)
Cereblon-BRD3 interaction.
Various amounts of ab271467 were incubated in 10-ul reaction with dBET1 and GST-BRD3(BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
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SDS-PAGE - Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) (ab271467)SDS-PAGE analysis of ab271467.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271467 は論文での使用が確認できていません。