Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Suitable for: SDS-PAGE, Functional Studies
製品名Recombinant Human Contactin 6 protein
Contactin 6 タンパク質・ペプチド 製品一覧
生理活性Induces Hes-1 in 3T3L-1 cells.
精製度> 90 % SDS-PAGE.
ab109345 is an 0.2 µm filtered solution.
エンドトキシン・レベル< 0.100 Eu/µg
発現系HEK 293 cells
タンパク質長Full length protein
予測される分子量150 kDa including tags
領域1 to 999
Our Abpromise guarantee covers the use of ab109345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
備考Induces Hes-1 in 3T3L-1 cells.
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- CNTN 6
機能Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination.
組織特異性Expressed in nervous system. Highly expressed in cerebellum. Expressed at intermediate level in thalamus, subthalamic nucleus. Weakly expressed in corpus callosum, caudate nucleus and spinal cord.
配列類似性Belongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
- Information by UniProt
Induction of Hes-1 with the treatment of hContactin-6-Fc.
A Mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 μg/ml of ab109345 as in indicated time points and each cell lysate was prepared and subjected to Western blot by using anti-Mouse Hes-1 or GAPDH (loading control).
Lane M: Marker
Lane 1: ab109345, 0 minutes
Lane 2: ab109345, 10 minutes
Lane 3: ab109345, 30 minutes
Lane 4: ab109345, 1 h
Lane 5: ab109345, 2 h
Lane 6: ab109345, 4 h
Lane 7: ab109345, 8 h
Lane 8: ab109345, 24 h
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.