製品名Recombinant human CDK3 + CCNE1 protein
タンパク質長Full length protein
由来Baculovirus infected Sf9 cells
Our Abpromise guarantee covers the use of ab85643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生理活性The Specific activity of ab85643 was determined to be 20 nmol/min/mg.
精製度> 90 % SDS-PAGE.
Purity was determined to be >90% by densitometry.
ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity
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Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- CCNE 1
関連性CDK3 is a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. CCNE1 (Cyclin E) is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
ab85643 has not yet been referenced specifically in any publications.