Recombinant human CD86 protein (Fc Chimera) (ab167720)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
製品の詳細
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製品名
Recombinant human CD86 protein (Fc Chimera)
CD86 タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its ability to induce IL2 secretion by Jurkat Human acute T cell leukemia cells. The ED50 for this effect is typically 0.04 - 0.2 ng/ml.
Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human ab167720 with a linear range of 2-78 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Mouse CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind ab167720 with a linear range of 5-78 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized ab167720 at 5 µg/mL (100 µL/well) can bind Biotinylated Human CTLA-4, Fc Tag, Avi Tag with a linear range of 0.6-10 ng/mL.
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精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
52 kDa -
領域
26 to 247 -
タグ
Fc tag C-Terminus -
配列の追加情報
Protein carries a human IgG1 Fc tag at the C-terminus. Due to glycosylation, the protein migrates as 66-86 kDa under reducing condition, and 120-140 kDa under non-reducing condition (SDS-PAGE).
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関連製品
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Recombinant Protein
特性
Our Abpromise guarantee covers the use of ab167720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
ELISA
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.75% Glycine, 0.48% Tris, L-Arginine, Sodium chloride
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成It is recommended to reconstitute the lyophilized protein with 500 µL sterile deionized water to a stock solution of 400 µg/mL.
関連情報
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別名
- Activation B7-2 antigen
- Activation B7-2 antigen 3
- B-lymphocyte activation antigen B7-2
see all -
機能
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
組織特異性
Expressed by activated B-lymphocytes and monocytes. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
細胞内局在
Membrane. - Information by UniProt
画像
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Human B7-2, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. The protein migrates as 66-86 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-PAGE).
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FACS assay shows that Human B7-2, Fc Tag can bind to 293T cells overexpressing human CTLA4 protein. The concentration of Human B7-2 is 3 µg/ml.
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FACS analysis shows that the binding of Human B7-2, Fc Tag to CD28 expressed on Jurkat E6.1 was inhibited by increasing concentration of neutralizing anti-CD28 antibody. The concentration of B7-2 used is 2 µg/ml. The IC50 is 0.022 µg/ml.
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Flow Cytometry assay shows that recombinant Human B7-2, Fc Tag can bind to CD28 expressed on Jurkat E6.1. The concentration of B7-2 used is 2 µg/ml(Routinely tested).
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Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human ab167720 with a linear range of 2-78 ng/mL.
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Immobilized Biotinylated Mouse CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind ab167720 with a linear range of 5-78 ng/mL.
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Immobilized ab167720 at 5 µg/mL (100 µL/well) can bind Biotinylated Human CTLA-4, Fc Tag, Avi Tag with a linear range of 0.6-10 ng/mL.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab167720 は 1 報の論文で使用されています。
- Wang J et al. Neuroprotective Effect of Fractalkine on Radiation-induced Brain Injury Through Promoting the M2 Polarization of Microglia. Mol Neurobiol 58:1074-1087 (2021). PubMed: 33089423