Recombinant human CD73 protein (Active) (Biotin) (ab271447)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, Avi tag C-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant human CD73 protein (Active) (Biotin)
CD73 タンパク質・ペプチド 製品一覧 -
生理活性
Specific activity >7000 pmol/min/μg.
Assay was done in 50 μL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 μM AMP for 15 min at 37°C. Phosphate standard solution was run side-by-side for product quantification.
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精製度
>= 90 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
WELTILHTND VHSRLEQTSE DSSKCVNASR CMGGVARLFT KVQQIRRAEP NVLLLDAGDQ YQGTIWFTVY KGAEVAHFMN ALRYDAMALG NHEFDNGVEG LIEPLLKEAK FPILSANIKA KGPLASQISG LYLPYKVLPV GDEVVGIVGY TSKETPFLSN PGTNLVFEDE ITALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DVVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTSDDGRKVP VVQAYAFGKY LGYLKIEFDE RGNVISSHGN PILLNSSIPE DPSIKADINK WRIKLDNYST QELGKTIVYL DGSSQSCRFR ECNMGNLICD AMINNNLRHT DEMFWNHVSM CILNGGGIRS PIDERNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDLSRKPG DRVVKLDVLC TKCRVPSYDP LKMDEVYKVI LPNFLANGGD GFQMIKDELL RHDSGDQDIN VVSTYISKMK VIYPAVEGRI K -
予測される分子量
61 kDa including tags -
領域
27 to 547 -
タグ
His tag C-Terminus , Avi tag C-Terminus -
配列の追加情報
Genbank: NM_002526
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標識
Biotin
関連製品
特性
Our Abpromise guarantee covers the use of ab271447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Liquid -
備考
Enzymatically biotin-labeled using Avi-tag™ technology
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- 5' NT
- 5' nucleotidase (CD73)
- 5' nucleotidase precursor
see all -
機能
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
関連疾患
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
配列類似性
Belongs to the 5'-nucleotidase family. -
細胞内局在
Cell membrane. - Information by UniProt
画像
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Functional Studies - Recombinant human CD73 protein (Active) (Biotin) (ab271447)
Functional analysis of ab271447.
Specific activity >7000 pmol/min/μg.
Assay was done in 50 μL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 μM AMP for 15 min at 37°C. Phosphate standard solution was run side-by-side for product quantification.
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SDS-PAGE - Recombinant Human CD73 protein (Tagged) (Biotin) (ab271447)SDS-PAGE analysis of ab271447.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271447 は論文での使用が確認できていません。